LOCUS       sequence-87527-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 513, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: YPet-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaaaggcg aagagctgtt
       61 caccggcgtg gtgcccatcc tggtggagct ggacggcgac gtgaacggcc acaagttcag
      121 cgtgagcggc gagggcgagg gcgacgccac ctacggcaag ctgaccctga agctgctgtg
      181 caccaccggc aagctgcccg tgccctggcc caccctggtg accaccctgg gctacggcgt
      241 gcagtgcttc gcccggtacc ccgaccacat gaagcagcac gacttcttca agagcgccat
      301 gcccgagggc tacgtgcagg agcggaccat cttcttcaag gacgacggca actacaagac
      361 ccgggccgag gtgaagttcg agggcgacac cctggtgaac cggatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg ccacaagctg gagtacaact acaacagcca
      481 caacgtgtac atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
      541 gcacaacatc gaggacggcg gcgtgcagct ggccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccaga gcgccctgtt
      661 caaggacccc aacgagaagc gggaccacat ggtgctgctg gagttcctga ccgccgccgg
      721 catcaccgag ggcatgaacg agctctataa gtccggactc agatctcgag tgcgtgagtg
      781 catctccatc cacgttggcc aggctggtgt ccagattggc aatgcctgct gggagctcta
      841 ctgcctggaa cacggcatcc agcccgatgg ccagatgcca agtgacaaga ccattggggg
      901 aggagatgac tccttcaaca ccttcttcag tgagacgggc gctggcaagc acgtgccccg
      961 ggctgt
//
