LOCUS       sequence-8753-ex        4314 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Kwon AR, Kessler BM, Overkleeft HS, McKay DB
  TITLE     Structure and reactivity of an asymmetric complex between HslV and 
            I-domain deleted HslU, a prokaryotic homolog of the eukaryotic 
            proteasome.
  JOURNAL   J Mol Biol. 2003 Jul 4. 330(2):185-95.
  PUBMED    12823960
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2003 Jul 4. 330(2):185-95."
COMMENT     Sequence Label: HI.HslU(delI)V.RSET.wt(Nde1).107-GG-244
FEATURES             Location/Qualifiers
     source          1..4314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1602..1620)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1616..1663
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1760..2215
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1847..1866)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2057..2078
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2296..2400
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2401..2469,2470..3261)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRPPRYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLID*MEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3432..4020
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3921..3940
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4174..4191
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4276..4295
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4276..4294
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctagaataat tttgtttaac tttaagaagg agatatacat atgacaacga ttgtaagcgt
       61 acgtcgcaat gggcaagtcg tcgtaggcgg tgatggacaa gtttctttag gcaataccgt
      121 aatgaaaggt aatgctcgta aagtacgccg tttatataat ggcaaagtgc ttgctggttt
      181 tgcgggtgga acggcagatg catttacact ttttgaatta tttgaacgta aacttgaaat
      241 gcatcaaggg catttgctaa aaagtgcggt ggaattagcg aaagattggc gtacagatcg
      301 cgcacttcgt aaattagaag cgatgctaat tgttgcggat gaaaaagaaa gtttaatcat
      361 cacgggtatt ggcgatgtgg ttcagcccga agaagatcaa attttagcaa tcggatcggg
      421 tggtaactat gcgttatctg cggcacgtgc gttagtggaa aatactgaac tttctgcaca
      481 tgaaattgtt gaaaaatctt taagaattgc tggtgatatt tgcgtgttca caaataccaa
      541 tttcactatt gaagaattac cgaattaagg aaaaacatat gtctgaaatg actcctcgcg
      601 aaatagtttc tgaattagat caacatatta ttggtcaagc cgatgcaaaa cgtgctgtgg
      661 cgattgcgtt gcgtaaccgt tggcgtcgta tgcagcttca agaacctttg cgtcatgaag
      721 taactccaaa aaatatttta atgattggcc caactggtgt gggtaaaact gaaattgcgc
      781 gccgtttagc aaaattagct aacgcaccat ttatcaaagt ggaagcaacg aaatttactg
      841 aagtaggtta cgtggggaaa gaggtggatt ccattattcg tgatttaacg gatagtgcgg
      901 gtggagccat tgatgctgtg gaacaaaatg gtatcgtctt tatcgatgaa atcgacaaaa
      961 tttgtaagaa aggtgaatac agtggtgctg atgtatcgcg tgaaggtgtg caacgtgatt
     1021 tacttccgtt agtagaaggc tcgacagtta gcaccaagca cggaatggtt aaaactgatc
     1081 atatcttatt tattgcatct ggtgcattcc aagttgctcg cccttcagat ttaattcctg
     1141 aactacaagg gcgtttgccg attcgtgttg aacttaccgc attaagtgcg gcggattttg
     1201 agcgtatttt aacagagcca catgcttctt taaccgaaca atataaagcg ttgatggcaa
     1261 cagaaggggt gaatattgca ttcacaacag atgcagtgaa gaaaattgct gaggcggcat
     1321 tccgagtgaa tgaaaaaacg gaaaatatcg gtgcacgccg tttgcatacg gtaatggagc
     1381 gtttgatgga taaaatttct ttcagcgcaa gcgacatgaa tggtcaaacg gtcaatattg
     1441 atgcagctta tgttgcggat gcacttggcg aagtggttga aaatgaagat ttgagccgtt
     1501 ttattcttta aagataaaaa ttttacgggt accgagctcg aattcgaagc ttgatccggc
     1561 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
     1621 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
     1681 atccggatct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     1741 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     1801 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     1861 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     1921 cctttagggt tccgatttag agctttacgg cacctcgacc gcaaaaaact tgatttgggt
     1981 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     2041 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatcgcg
     2101 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     2161 ctgatttaac aaatatttaa cgcgaatttt aacaaaatat taacgtttac aatttcgcct
     2221 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac aggtggcact
     2281 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2341 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2401 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2461 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     2521 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     2581 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtgatac actattatcc
     2641 cgtattgacg ccgggcaaga gcaactcggt cgccccccgc ggtattctca gaatgacttg
     2701 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     2761 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     2821 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     2881 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagagtga caccacgatg
     2941 cctgtagcaa tgccaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3001 tcccggcaac aattaataga ctgaatggag gcggataaag ttgcaggacc acttctgcgc
     3061 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     3121 cgcggtatca ttgcagcact ggggccagat ggtaagcgct cccgtatcgt agttatctac
     3181 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     3241 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     3301 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     3361 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3421 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3481 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3541 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     3601 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3661 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3721 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3781 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3841 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3901 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3961 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4021 aacgccagca acgcggcctt tttacggttc ctgggctttt gctggccttt tgctcacatg
     4081 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     4141 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     4201 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcaggatc
     4261 tcgatcccgc gaaattaata cgactcacta tagggagacc acaacggttt ccct
//
