LOCUS       sequence-87532-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..56
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     7..27
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..811
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSFLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMYLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
       61 tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc atggtgagca agggcgaggg
      121 gcaatcgaag catgggctaa aggaggagat gacggtgaag taccacatgg aaggttgcgt
      181 taatggtcac aaatttgtca ttactggaga gggcattgga aaccctttta agggtaagca
      241 aactgcaaat ttgtgtgtga tagaaggagg cccgctgccg ttctcggagg acattttaag
      301 tccgggtttt aaatatggtg accggatttt cacagagtac ccacaggata ttgtagatta
      361 cttcaagaac tcatgtccag cgggctatac gtgggaaagg agcttcctct ttgaggacgg
      421 agcggtctgt cgatgcaacg tggacataac agtctctgaa aaggagaact gcatctatca
      481 caaaagtatc ttcagagggg tgaattttcc cgccgacggc cccgtaatga agaaaatgac
      541 cactaattgg gaagctagta ccgagaaaat tgtgcctgtt ccaaagcaag ggatattaaa
      601 aggaaaggtc aaaatgtacc tattgctgaa ggatggcggt cgttatcatt gccagtttga
      661 tacggtatat aaagctaaat cagtgccctc caagatgcca gaatggcatt ttatacagca
      721 taagctcctt agggaggacc gctctgatgc taaaaaccag aaatggcaat taacagaaca
      781 tgcaatcgca ggcatggacg agctgtacaa gtccggactc agatctaagc tgaaccctcc
      841 tgatgagagt ggccccggct gcatgagctg caagtgtgtg ctctcctgag aattctgcag
      901 tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata
      961 ccacatttgt agaggtttta cttgctttaa aaaa
//
