LOCUS       sequence-87534-e        1494 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya-Rab4a-7
FEATURES             Location/Qualifiers
     source          1..1494
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..756
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSFLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMYLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 ccatgcttag tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc
       61 gaggggcaat cgaagcatgg gctaaaggag gagatgacgg tgaagtacca catggaaggt
      121 tgcgttaatg gtcacaaatt tgtcattact ggagagggca ttggaaaccc ttttaagggt
      181 aagcaaactg caaatttgtg tgtgatagaa ggaggcccgc tgccgttctc ggaggacatt
      241 ttaagtccgg gttttaaata tggtgaccgg attttcacag agtacccaca ggatattgta
      301 gattacttca agaactcatg tccagcgggc tatacgtggg aaaggagctt cctctttgag
      361 gacggagcgg tctgtcgatg caacgtggac ataacagtct ctgaaaagga gaactgcatc
      421 tatcacaaaa gtatcttcag aggggtgaat tttcccgccg acggccccgt aatgaagaaa
      481 atgaccacta attgggaagc tagtaccgag aaaattgtgc ctgttccaaa gcaagggata
      541 ttaaaaggaa aggtcaaaat gtacctattg ctgaaggatg gcggtcgtta tcattgccag
      601 tttgatacgg tatataaagc taaatcagtg ccctccaaga tgccagaatg gcattttata
      661 cagcataagc tccttaggga ggaccgctct gatgctaaaa accagaaatg gcaattaaca
      721 gaacatgcaa tcgcaggcat ggacgagctg tacaagtccg gactcagatc tcgagccatg
      781 tcgcagacgg ccatgtccga aacctacgat tttttgttta agttcttggt tattggaaat
      841 gcaggaactg gcaaatcttg cttacttcat cagtttattg aaaaaaaatt caaagatgac
      901 tcaaatcata caataggagt ggaatttggt tcaaagataa taaatgttgg tggtaaatat
      961 gtaaagttac aaatatggga tacagcagga caagaacgat tcaggtccgt gacgagaagt
     1021 tattaccgag gcgcggccgg ggctctcctc gtctatgata tcaccagccg agaaacctac
     1081 aatgcgctta ctaattggtt aacagatgcc cgaatgctgg cgagccagaa cattgtgatc
     1141 atcctttgtg gaaacaagaa ggacctggat gcagatcgtg aagttacctt cttagaagcc
     1201 tccagatttg ctcaagaaaa tgagctgatg tttttggaaa caagtgcgct cacaggggag
     1261 aatgtagaag aggcttttgt acagtgtgca agaaaaatac ttaacaaaat cgaatcaggt
     1321 gagctggacc cagaaagaat gggctcaggt attcagtacg gagatgctgc cttgagacag
     1381 ctgaggtcac cgcggcgcgc acaggccccg aacgctcagg agtgtggttg ttagggatcc
     1441 accggatcta gataactgat cataatcagc cataccacat gtagaagata gccc
//
