LOCUS       sequence-87536-e        2534 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..2534
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1778..1787
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1784..2497
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSFLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMYLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattcca tggcggcccc
       61 ccgcccgtct cccgcgatcc ccgtttcggt ctcggctccg gctttttacg ccccgcagaa
      121 gaagttcggc cctgtggtgg ccccaaagcc caaagtgaat cccttccggc ccagggacag
      181 cgagcctccc ccggcacccg gggcccagcg cgcacagatg ggccgggtgg gcgatattcc
      241 cccgccgccc ccggaagact ttcccctgcc tccacctccc cttgctgggg atggcgacga
      301 tgcagagggt gctctgggag gtgccttccc gccgccccct cccccgatcg aggaatcatt
      361 tccccctgcg cctctggagg aggagatctt cccttccccg ccgcctcctc cggaggagga
      421 gggagggcct gaggccccca taccgccccc accacagccc agggagaagg tgagcagtat
      481 tgatttggag atcgactctc tgtcctcact gctggatgac atgaccaaga atgatccttt
      541 caaagcccgg gtgtcatctg gatatgtgcc cccaccagtg gccactccat tcagttccaa
      601 gtccagtacc aagcctgcag ccgggggcac agcacccctg cctccttgga agtccccttc
      661 cagctcccag cctctgcccc aggttccggc tccggctcag agccagacac agttccatgt
      721 tcagccccag ccccagccca agcctcaggt ccaactccat gtccagtccc agacccagcc
      781 tgtgtctttg gctaacaccc agccccgagg gcccccagcc tcatctccgg ctccagcccc
      841 taagttttct ccagtgactc ctaagtttac tcctgtggct tccaagttca gtcctggagc
      901 cccaggtgga tctgggtcac aaccaaatca aaaattgggg caccccgaag ctctttctgc
      961 tggcacaggc tcccctcaac ctcccagctt cacctatgcc cagcagaggg agaagccccg
     1021 agtgcaggag aagcagcacc ccgtgccccc accggctcag aaccaaaacc aggtgcgctc
     1081 ccctggggcc ccagggcccc tgactctgaa ggaggtggag gagctggagc agctgaccca
     1141 gcagctaatg caggacatgg agcatcctca gaggcagaat gtggctgtca acgaactctg
     1201 cggccgatgc catcaacccc tggcccgggc gcagccagcc gtccgcgctc tagggcagct
     1261 gttccacatc gcctgcttca cctgccacca gtgtgcgcag cagctccagg gccagcagtt
     1321 ctacagtctg gagggggcgc cgtactgcga gggctgttac actgacaccc tggagaagtg
     1381 taacacctgc ggggagccca tcactgaccg catgctgagg gccacgggca aggcctatca
     1441 cccgcactgc ttcacctgtg tggtctgcgc ccgccccctg gagggcacct ccttcatcgt
     1501 ggaccaggcc aaccggcccc actgtgtccc cgactaccac aagcagtacg ccccgaggtg
     1561 ctccgtctgc tctgagccca tcatgcctga gcctggccga gatgagactg tgcgagtggt
     1621 cgccctggac aagaacttcc acatgaagtg ttacaagtgt gaggactgcg ggaagcccct
     1681 gtcgattgag gcagatgaca atggctgctt ccccctggac ggtcacgtgc tctgtcggaa
     1741 gtgccacact gctagagccc agacggatcc accggtcgcc accatggtga gcaagggcga
     1801 ggggcaatcg aagcatgggc taaaggagga gatgacggtg aagtaccaca tggaaggttg
     1861 cgttaatggt cacaaatttg tcattactgg agagggcatt ggaaaccctt ttaagggtaa
     1921 gcaaactgca aatttgtgtg tgatagaagg aggcccgctg ccgttctcgg aggacatttt
     1981 aagtccgggt tttaaatatg gtgaccggat tttcacagag tacccacagg atattgtaga
     2041 ttacttcaag aactcatgtc cagcgggcta tacgtgggaa aggagcttcc tctttgagga
     2101 cggagcggtc tgtcgatgca acgtggacat aacagtctct gaaaaggaga actgcatcta
     2161 tcacaaaagt atcttcagag gggtgaattt tcccgccgac ggccccgtaa tgaagaaaat
     2221 gaccactaat tgggaagcta gtaccgagaa aattgtgcct gttccaaagc aagggatatt
     2281 aaaaggaaag gtcaaaatgt acctattgct gaaggatggc ggtcgttatc attgccagtt
     2341 tgatacggta tataaagcta aatcagtgcc ctccaagatg ccagaatggc attttataca
     2401 gcataagctc cttagggagg accgctctga tgctaaaaac cagaaatggc aattaacaga
     2461 acatgcaatc gcaggcatgg acgagctgta caagtaagcg gccgcgactc tagatcataa
     2521 tcagccatac caca
//
