LOCUS       sequence-87541-e        1455 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya1-Rab4a-7
FEATURES             Location/Qualifiers
     source          1..1455
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..740
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSYLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMCLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 cagatccgct agcgctaccg gtcgccacca tggtgagcaa gggcgagggg caatcgaagc
       61 atggactaaa ggaggagatg acggtgaagt accacatgga aggttgcgtt aatggtcaca
      121 aatttgtcat tactggagag ggcattggaa acccttttaa gggtaagcaa accgcaaatt
      181 tgtgtgtgat agaaggaggc ccgctgccgt tctcggagga cattttaagt ccgggtttta
      241 aatatggtga ccggattttc acagagtacc cacaggatat tgtagattac ttcaagaact
      301 catgtccagc gggctatacg tgggaaagga gctacctctt tgaggacgga gcggtctgtc
      361 gatgcaacgt ggacataaca gtctctgaaa aggagaactg catctatcac aaaagtatct
      421 tcagaggggt gaattttccc gccgacggcc ccgtaatgaa gaaaatgacc actaattggg
      481 aagctagtac cgagaaaatt gtgcctgttc caaagcaagg gatattaaag ggaaaggtca
      541 aaatgtgcct gttgctgaag gatggcggtc gttatcattg ccagtttgat acggtatata
      601 aagctaaatc agtgccctcc aagatgccag aatggcattt tatacagcat aagctcctta
      661 gggaggaccg ctctgatgct aaaaaccaga aatggcaatt aacagaacat gcaatcgcag
      721 gcatggacga gctgtacaag tccggactca gatctcgagc catgtcgcag acggccatgt
      781 ccgaaaccta cgattttttg tttaagttct tggttattgg aaatgcagga actggcaaat
      841 cttgcttact tcatcagttt attgaaaaaa aattcaaaga tgactcaaat catacaatag
      901 gagtggaatt tggttcaaag ataataaatg ttggtggtaa atatgtaaag ttacaaatat
      961 gggatacagc aggacaagaa cgattcaggt ccgtgacgag aagttattac cgaggcgcgg
     1021 ccggggctct cctcgtctat gatatcacca gccgagaaac ctacaatgcg cttactaatt
     1081 ggttaacaga tgcccgaatg ctggcgagcc agaacattgt gatcatcctt tgtggaaaca
     1141 agaaggacct ggatgcagat cgtgaagtta ccttcttaga agcctccaga tttgctcaag
     1201 aaaatgagct gatgtttttg gaaacaagtg cgctcacagg ggagaatgta gaagaggctt
     1261 ttgtacagtg tgcaagaaaa atacttaaca aaatcgaatc aggtgagctg gacccagaaa
     1321 gaatgggctc aggtattcag tacggagatg ctgccttgag acagctgagg tcaccgcggc
     1381 gcgcacaggc cccgaacgct caggagtgtg gttgttaggg atccaccgga tctagataac
     1441 tgatcataat cagcc
//
