LOCUS       sequence-87542-e        2163 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya1-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..2163
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..743
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSYLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMCLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gggcaatcga
       61 agcatggact aaaggaggag atgacggtga agtaccacat ggaaggttgc gttaatggtc
      121 acaaatttgt cattactgga gagggcattg gaaacccttt taagggtaag caaaccgcaa
      181 atttgtgtgt gatagaagga ggcccgctgc cgttctcgga ggacatttta agtccgggtt
      241 ttaaatatgg tgaccggatt ttcacagagt acccacagga tattgtagat tacttcaaga
      301 actcatgtcc agcgggctat acgtgggaaa ggagctacct ctttgaggac ggagcggtct
      361 gtcgatgcaa cgtggacata acagtctctg aaaaggagaa ctgcatctat cacaaaagta
      421 tcttcagagg ggtgaatttt cccgccgacg gccccgtaat gaagaaaatg accactaatt
      481 gggaagctag taccgagaaa attgtgcctg ttccaaagca agggatatta aagggaaagg
      541 tcaaaatgtg cctgttgctg aaggatggcg gtcgttatca ttgccagttt gatacggtat
      601 ataaagctaa atcagtgccc tccaagatgc cagaatggca ttttatacag cataagctcc
      661 ttagggagga ccgctctgat gctaaaaacc agaaatggca attaacagaa catgcaatcg
      721 caggcatgga cgagctgtac aagtccggac tcagatctcg agtgcgtgag tgcatctcca
      781 tccacgttgg ccaggctggt gtccagattg gcaatgcctg ctgggagctc tactgcctgg
      841 aacacggcat ccagcccgat ggccagatgc caagtgacaa gaccattggg ggaggagatg
      901 actccttcaa caccttcttc agtgagacgg gcgctggcaa gcacgtgccc cgggctgtgt
      961 ttgtagactt ggaacccaca gtcattgatg aagttcgcac tggcacctac cgccagctct
     1021 tccaccctga gcagctcatc acaggcaagg aagatgctgc caataactat gcccgagggc
     1081 actacaccat tggcaaggag atcattgacc ttgtgttgga ccgaattcgc aagctggctg
     1141 accagtgcac cggtcttcag ggcttcttgg ttttccacag ctttggtggg ggaactggtt
     1201 ctgggttcac ctccctgctc atggaacgtc tctcagttga ttatggcaag aagtccaagc
     1261 tggagttctc catttaccca gcaccccagg tttccacagc tgtagttgag ccctacaact
     1321 ccatcctcac cacccacacc accctggagc actctgattg tgccttcatg gtagacaatg
     1381 aggccatcta tgacatctgt cgtagaaacc tcgatatcga gcgcccaacc tacactaacc
     1441 ttaaccgcct tattagccag attgtgtcct ccatcactgc ttccctgaga tttgatggag
     1501 ccctgaatgt tgacctgaca gaattccaga ccaacctggt gccctacccc cgcatccact
     1561 tccctctggc cacatatgcc cctgtcatct ctgctgagaa agcctaccat gaacagcttt
     1621 ctgtagcaga gatcaccaat gcttgctttg agccagccaa ccagatggtg aaatgtgacc
     1681 ctcgccatgg taaatacatg gcttgctgcc tgttgtaccg tggtgacgtg gttcccaaag
     1741 atgtcaatgc tgccattgcc accatcaaaa ccaagcgcag catccagttt gtggattggt
     1801 gccccactgg cttcaaggtt ggcatcaact accagcctcc cactgtggtg cctggtggag
     1861 acctggccaa ggtacagaga gctgtgtgca tgctgagcaa caccacagcc attgctgagg
     1921 cctgggctcg cctggaccac aagtttgacc tgatgtatgc caagcgtgcc tttgttcact
     1981 ggtacgtggg tgaggggatg gaggaaggcg agttttcaga ggcccgtgaa gatatggctg
     2041 cccttgagaa ggattatgag gaggttggtg tggattctgt tgaaggagag ggtgaggaag
     2101 aaggagagga atactaagga tccaccggat ctagataact gatcataatc agccatacca
     2161 cat
//
