LOCUS       sequence-87544-e        2523 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya1-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..2523
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      44..53
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1774..1783
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1780..2493
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSYLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMCLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 gctagcgcta ccggactcag atctcgagct caagcttcga attccatggc ggccccccgc
       61 ccgtctcccg cgatccccgt ttcggtctcg gctccggctt tttacgcccc gcagaagaag
      121 ttcggccctg tggtggcccc aaagcccaaa gtgaatccct tccggcccag ggacagcgag
      181 cctcccccgg cacccggggc ccagcgcgca cagatgggcc gggtgggcga tattcccccg
      241 ccgcccccgg aagactttcc cctgcctcca cctccccttg ctggggatgg cgacgatgca
      301 gagggtgctc tgggaggtgc cttcccgccg ccccctcccc cgatcgagga atcatttccc
      361 cctgcgcctc tggaggagga gatcttccct tccccgccgc ctcctccgga ggaggaggga
      421 gggcctgagg cccccatacc gcccccacca cagcccaggg agaaggtgag cagtattgat
      481 ttggagatcg actctctgtc ctcactgctg gatgacatga ccaagaatga tcctttcaaa
      541 gcccgggtgt catctggata tgtgccccca ccagtggcca ctccattcag ttccaagtcc
      601 agtaccaagc ctgcagccgg gggcacagca cccctgcctc cttggaagtc cccttccagc
      661 tcccagcctc tgccccaggt tccggctccg gctcagagcc agacacagtt ccatgttcag
      721 ccccagcccc agcccaagcc tcaggtccaa ctccatgtcc agtcccagac ccagcctgtg
      781 tctttggcta acacccagcc ccgagggccc ccagcctcat ctccggctcc agcccctaag
      841 ttttctccag tgactcctaa gtttactcct gtggcttcca agttcagtcc tggagcccca
      901 ggtggatctg ggtcacaacc aaatcaaaaa ttggggcacc ccgaagctct ttctgctggc
      961 acaggctccc ctcaacctcc cagcttcacc tatgcccagc agagggagaa gccccgagtg
     1021 caggagaagc agcaccccgt gcccccaccg gctcagaacc aaaaccaggt gcgctcccct
     1081 ggggccccag ggcccctgac tctgaaggag gtggaggagc tggagcagct gacccagcag
     1141 ctaatgcagg acatggagca tcctcagagg cagaatgtgg ctgtcaacga actctgcggc
     1201 cgatgccatc aacccctggc ccgggcgcag ccagccgtcc gcgctctagg gcagctgttc
     1261 cacatcgcct gcttcacctg ccaccagtgt gcgcagcagc tccagggcca gcagttctac
     1321 agtctggagg gggcgccgta ctgcgagggc tgttacactg acaccctgga gaagtgtaac
     1381 acctgcgggg agcccatcac tgaccgcatg ctgagggcca cgggcaaggc ctatcacccg
     1441 cactgcttca cctgtgtggt ctgcgcccgc cccctggagg gcacctcctt catcgtggac
     1501 caggccaacc ggccccactg tgtccccgac taccacaagc agtacgcccc gaggtgctcc
     1561 gtctgctctg agcccatcat gcctgagcct ggccgagatg agactgtgcg agtggtcgcc
     1621 ctggacaaga acttccacat gaagtgttac aagtgtgagg actgcgggaa gcccctgtcg
     1681 attgaggcag atgacaatgg ctgcttcccc ctggacggtc acgtgctctg tcggaagtgc
     1741 cacactgcta gagcccagac ggatccaccg gtcgccacca tggtgagcaa gggcgagggg
     1801 caatcgaagc atggactaaa ggaggagatg acggtgaagt accacatgga aggttgcgtt
     1861 aatggtcaca aatttgtcat tactggagag ggcattggaa acccttttaa gggtaagcaa
     1921 accgcaaatt tgtgtgtgat agaaggaggc ccgctgccgt tctcggagga cattttaagt
     1981 ccgggtttta aatatggtga ccggattttc acagagtacc cacaggatat tgtagattac
     2041 ttcaagaact catgtccagc gggctatacg tgggaaagga gctacctctt tgaggacgga
     2101 gcggtctgtc gatgcaacgt ggacataaca gtctctgaaa aggagaactg catctatcac
     2161 aaaagtatct tcagaggggt gaattttccc gccgacggcc ccgtaatgaa gaaaatgacc
     2221 actaattggg aagctagtac cgagaaaatt gtgcctgttc caaagcaagg gatattaaag
     2281 ggaaaggtca aaatgtgcct gttgctgaag gatggcggtc gttatcattg ccagtttgat
     2341 acggtatata aagctaaatc agtgccctcc aagatgccag aatggcattt tatacagcat
     2401 aagctcctta gggaggaccg ctctgatgct aaaaaccaga aatggcaatt aacagaacat
     2461 gcaatcgcag gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag
     2521 cca
//
