LOCUS       sequence-87548-e        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI dy C-D
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(121..137)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(121..137)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(134..156)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    145..161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(169..175,176..193,194..199))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    214..235
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(352..369)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(603..622)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctcgcgaat gcatctagat atcggatccc tttggtctct cacctgacct aagctagcct
       61 agaaggtgag accgggcccg tcgactgcag aggcctgcat gcaagcttgg cgtaatcatg
      121 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      181 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      241 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      301 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
      361 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      421 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      481 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      541 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      601 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      661 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      721 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      781 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      841 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      901 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      961 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1021 tagctcttga tccggcaa
//
