LOCUS       sequence-87550-e        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI dy C-D
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(124..141)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(124..140)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(133..155)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(349..368)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     468..490
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(528..546)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agcttgcatg caggcctctg cagtcgacgg gcccggtctc accttctagg ctagcttagg
       61 tcaggtgaga gaccaaaggg atccgatatc tagatgcatt cgcgaggtac cgagctcgaa
      121 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      181 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      241 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
      301 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
      361 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
      421 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accttctccg ggagctgcat
      481 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga caaaagggcc tcgtgatacg
      541 cctattttta taggttaatg tcatgagcgg cgttgtgaca atttaccgaa caactccgcg
      601 gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca
      661 aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca
      721 ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc atgcttgagg
      781 agattgatga gcgcggtggc aatgccctgc ctccggtgct ctccggagac tgcgagatca
      841 tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc cgcgagagcg
      901 ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag
      961 ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc gccgaactca
     1021 cgaccgaaaa gatcaagagc agcccgcatg gatt
//
