LOCUS       sequence-87553-e        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI dy D-E
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(101..118)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(101..117)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(110..132)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(326..345)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     445..467
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(505..523)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agcttgcagg tctcagattc taggctagct taggtcttag cccttagaga cctagggatc
       61 cgatatctag atgcattcgc gaggtaccga gctcgaattc actggccgtc gttttacaac
      121 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      181 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      241 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
      301 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
      361 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
      421 cttacagaca agctgtgacc ttctccggga gctgcatgtg tcagaggttt tcaccgtcat
      481 caccgaaacg cgcgagacaa aagggcctcg tgatacgcct atttttatag gttaatgtca
      541 tgagcggcgt tgtgacaatt taccgaacaa ctccgcggcc gggaagccga tctcggcttg
      601 aacgaattgt taggtggcgg tacttgggtc gatatcaaag tgcatcactt cttcccgtat
      661 gcccaacttt gtatagagag ccactgcggg atcgtcaccg taatctgctt gcacgtagat
      721 cacataagca ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg cggtggcaat
      781 gccctgcctc cggtgctctc cggagactgc gagatcatag atatagatct cactacgcgg
      841 ctgctcaaac ttgggcagaa cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa
      901 ggcagcaagc gcgatgaatg tcttactacg gagcaagttc ccgaggtaat cggagtccgg
      961 ctgatgttgg gagtaggtgg ctacgtcgcc gaactcacga ccgaaaagat caaga
//
