LOCUS       sequence-87555-e         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI E-F 35S term c1
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(363..379)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(363..379)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(376..398)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    387..403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(411..417,418..435,436..441))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    456..477
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(594..611)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(845..864)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agctcggtac ctcgatgaag acttcagagg tctcactcaa ggtcactgga ttttggtttt
       61 aggaattaga aattttattg atagaagtat tttacaaata caaatacata ctaagggttt
      121 cttatatgct caacacatga gcgaaaccct ataagaaccc taattccctt atctgggaac
      181 tactcacaca ttattctgga gaaaaataga gagagataga tttgtagaga gagactggtg
      241 atttttgcgg actctagcat ggccggattt gagacccgta aagtcttcat cgaatgcatc
      301 tagatatcgg atcccgggcc cgtcgactgc agaggcctgc atgcaagctt ggcgtaatca
      361 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      421 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      481 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
      541 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
      601 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
      661 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      721 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      781 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      841 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      901 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
      961 agctcacgct gtag
//
