LOCUS       sequence-87563-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     Scott Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUAST flag Rab23 WT
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             59..82
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    143..167
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agagactctg aatagggaat tgggaattcg ttaacagatc tgcggccgcg ccaccatgga
       61 ttacaaggat gacgacgata agagatctcg agctcaagct tcgaattctg cagtcgacgg
      121 taccgcgggc ccgggatcat caacaagttt gtacaaaaaa gcaggcttaa tgcgtctaat
      181 ccaaacggca accggcggtg ctgctgcgtc cgttctgcag acccactccc aggcgcagta
      241 caactacacc agcatgcgtg aagatgatat cgagctggcc attaaagtgg tcattgttgg
      301 aaatggcggc gttggcaagt catcaatgat ccagcgctat tgcaaaggca ttttcacgaa
      361 ggactacaag aaaacgattg gagtggactt cctagagcga cagatcgaga tcgacggcga
      421 ggatgtgcgc atcatgctgt gggacaccgc cggccaggag gagttcgact gcatcacaaa
      481 ggcgtactac cgcggcgccc aggcttccgt cctggtcttt tcgacgacgg atcgagcatc
      541 cttcgacgcg atcaaggatt ggaagcgcaa ggtggagaac gagtgcaacg agatacccac
      601 agtgattgtg cagaacaaga tcgatcttat cgaacaggcg gtggttacgg ccgacgaggt
      661 ggagacgctg gccaagctcc tcaactgccg gctcatccgc acctccgtca aggaggacat
      721 taacgtggcg tccgtgtttc gctacctggc caccaagtgc catcagctaa tgacccagag
      781 ctacgaccag gtggccggaa accagcaaaa ctccagccat ccgccttaca gcagcacgcc
//
