LOCUS       sequence-87569-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI D-E Cerulean + linker c5
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(30..743,744..746))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(75..96)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     424..443
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     683..704
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(875..892)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(875..891)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(884..906)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agcttgcatg caggcagagg tctcagattt tacttgtaca gctcgtccat gccgagagtg
       61 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
      121 ttgctcagct tggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
      181 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
      241 tggcggatct tgaagttggc cttgatgccg ttcttctgct tgtcggcggt gatatagacg
      301 ttgtcgctga tggcgttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
      361 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
      421 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg
      481 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac
      541 tgcacgcccc aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
      601 gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac
      661 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
      721 gtgaacagct cctcgccctt gctcacgcta cctccgccac cacttccacc gcctccagaa
      781 cctcctccac ctccctttga gacccgtacc tctgcagtcg acgggcccgg gatccgatat
      841 ctagatgcat tcgcgaggta ccgagctcga attcactggc cgtcgtttta caacgtcgtg
      901 actgggaaaa ccctggcgtt acccaactta atcgccttgc a
//
