LOCUS       sequence-87573-e        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI C-D mOrange c3
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             77..784
                     /codon_start=1
                     /product="orange monomeric derivative of DsRed fluorescent 
                     protein (Shanet et al., 2004)"
                     /label=mOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(827..843)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(827..843)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(840..862)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    851..867
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(875..881,882..899,900..905))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    920..941
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgagctcggt acctcgcgaa tgcatctaga tatcggatcc cgggcccgtc gactgcagag
       61 gtacgggtct cacaccatgg tgagcaaggg cgaggagaat aacatggcca tcatcaagga
      121 gttcatgcgc ttcaaggtgc gcatggaggg ctctgtgaac ggccacgagt tcgagatcga
      181 gggcgagggc gagggccgcc cctacgaggg ctttcagacc gctaagctga aggtgaccaa
      241 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcacct acggctccaa
      301 ggcctacgtg aagcaccccg ccgacatccc cgactacttc aagctgtcct tccccgaggg
      361 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
      421 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
      481 ctccgacggc cccgtaatgc agaagaaaac catgggctgg gaggcctcct ccgagcggat
      541 gtaccccgag gacggcgccc tgaagggcga gatcaagatg aggctgaagc tgaaggacgg
      601 cggccactac acctccgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
      661 cggcgcctac atcgtcggca tcaagttgga catcacctcc cacaacgagg actacaccat
      721 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
      781 caagaaggtg agacctctgc ctgcatgcaa gcttggcgta atcatggtca tagctgtttc
      841 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
      901 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      961 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     1021 ggagaggcgg tttgcgtat
//
