LOCUS       sequence-87575-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI C-D mOrange c3
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..737)
                     /codon_start=1
                     /product="orange monomeric derivative of DsRed fluorescent 
                     protein (Shanet et al., 2004)"
                     /label=mOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(819..836)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(819..835)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(828..850)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agcttgcatg caggcagagg tctcaccttc ttgtacagct cgtccatgcc gccggtggag
       61 tggcggccct cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg
      121 atgtccaact tgatgccgac gatgtaggcg ccgggcagct gcacgggctt cttggccttg
      181 taggtggtct tgacctcgga ggtgtagtgg ccgccgtcct tcagcttcag cctcatcttg
      241 atctcgccct tcagggcgcc gtcctcgggg tacatccgct cggaggaggc ctcccagccc
      301 atggttttct tctgcattac ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc
      361 ttgtagatga actcgccgtc ctgcagggag gagtcctggg tcacggtcac cacgccgccg
      421 tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttgaagtag
      481 tcggggatgt cggcggggtg cttcacgtag gccttggagc cgtaggtgaa ctgaggggac
      541 aggatgtccc aggcgaaggg cagggggcca cccttggtca ccttcagctt agcggtctga
      601 aagccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcaca
      661 gagccctcca tgcgcacctt gaagcgcatg aactccttga tgatggccat gttattctcc
      721 tcgcccttgc tcaccatggt gtgagacccg tacctctgca gtcgacgggc ccgggatccg
      781 atatctagat gcattcgcga ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt
      841 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      901 gccagctggc gtaata
//
