LOCUS       sequence-87596-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Lopez ME, Klein AD, Dimbil UJ, Scott MP
  TITLE     Anatomically defined neuron-based rescue of neurodegenerative 
            Niemann-Pick type C disorder.
  JOURNAL   J Neurosci. 2011 Mar 23;31(12):4367-78. doi: 
            10.1523/JNEUROSCI.5981-10.2011.
  PUBMED    21430138
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.5981-10"; journalName: "J Neurosci"; date: 
            "2011-03-23- 23"; volume: "31"; issue: "12"; pages: "4367-78"
COMMENT     Sequence Label: NPC1 His6 EYFP
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(53..70)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga tggatcccgg gcccgcggta ccgtggtggt
       61 ggtggtggtg aaaattgagg agccgttctc tctctgtccc tctgtagcgc tcgtaagtgg
      121 tgtgtctttt agctttattc accgacggcc ctatgtaact gagtaagacg ggaagaaata
      181 ttagtccatg agtggctccg agtaagacca tggctaagta catcctgaag taaaatatct
      241 caaaaatttg agatttggca aaggctaaca ccacgatccc tccaaatttc gtaagtgtga
      301 ttccactgaa tacagaacta cccatgtggg ccagtgcctc ttccgcccgg ctcactcggc
      361 ttcctttggt actcatggtg aatgctctcg ttatatggct gcagaactcc acagaaatgc
      421 cgcagctcat caccaagttg accaaggaga ccgcattcag actgatgccc cacagccaca
      481 tgacaccgaa catgttgacc aggatcatgg ctatggtgat acacatgatg accgcagacc
      541 acagctcaca gcccagaacc accaaggtca ccagaaatat ggagcccaga gacacactga
      601 ggttaaagat ggtgtcatca ataatggtca ggtactgttc atagaagacg tagaacacac
      661 tgtaagggaa tacgcggtag tcactcccct tagaacgcat ggtttccgtg atgttactgg
      721 ctattagccg agctttcttc atggcatcag tatagtcagc ggaggtctta agtatggtgt
      781 ggtaggtcat gaagtaagtg gccccaatgt aagtgtcatc tcccacaatg ttaacagctg
      841 aacc
//
