LOCUS       sequence-87597-e         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty vector to express N-terminal His-mOrange-tagged fusion 
            protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Tome JM, Ozer A, Pagano JM, Gheba D, Schroth GP, Lis JT
  TITLE     Comprehensive analysis of RNA-protein interactions by 
            high-throughput sequencing-RNA affinity profiling.
  JOURNAL   Nat Methods. 2014 Jun;11(6):683-8. doi: 10.1038/nmeth.2970. Epub 
            2014 May 8.
  PUBMED    24809628
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2970"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "683-8"
COMMENT     Sequence Label: mOrange pHis-//
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..60
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(113..130)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(573..592)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aaaaacccct caagacccgt ttagaggccc caaggggtta tgctagttat tgctcagcgg
       61 tggcagcagc caactcagct tcctttcggg ctttgttagc agccggatct cagtggtggt
      121 ggtggtggtg ctcgagtgcg gccgcaagct tggtaccgca tgcctcgaga ctgcaggctc
      181 tagattcgaa agcggccgca ctagttgagc tcgtcgacgt aggcctttga attccggatc
      241 cgacgaacct ccacctcctg atccgcctcc acctgatccg cctccacctg atccaccctt
      301 gtacagctcg tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt actgttccac
      361 gatggtgtag tcctcgttgt gggaggtgat gtccaacttg atgccgacga tgtaggcgcc
      421 gggcagctgc acgggcttct tggccttgta ggtggtcttg acctcggagg tgtagtggcc
      481 gccgtccttc agcttcagcc tcatcttgat ctcgcccttc agggcgccgt cctcggggta
      541 catccgctcg gaggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcgga
      601 ggggaagttg gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct ggagggagga
      661 gtcctgggtc acggtcacca cgccgcc
//
