LOCUS       sequence-87598-e        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI B-C GFP
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(79..81,82..84,85..795)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(124..145)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(385..404)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     732..753
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(885..901)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(885..901)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(898..920)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    909..925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(933..939,940..957,958..963))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    978..999
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 agctcggtac ctcgcgaatg catctagata tcggatcccg ggcccgtcga ctgcagaggt
       61 acgggtctca tctgaacaat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      121 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      181 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      241 cccgtgccct ggcccaccct cgtgaccacc ttcacctacg gcgtgcagtg cttcagccgc
      301 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      361 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      421 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      481 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
      541 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
      601 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      661 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
      721 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tcacggcatg
      781 gacgagctgt acaagggtgg aggaggttct ggaggcggtg gaagtggtgg cggaggtagc
      841 ggcacctgag acctctgcct gcatgcaagc ttggcgtaat catggtcata gctgtttcct
      901 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      961 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     1021 gctttccagt c
//
