LOCUS       sequence-87603-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI dy B-C
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(110..127)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(110..126)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(119..141)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(335..354)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     454..476
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(514..532)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agcttgcatg caggcctctg cagtcgacgg gcccggtctc aggtgcttcc agaagagacc
       61 aaagggatcc gatatctaga tgcattcgcg aggtaccgag ctcgaattca ctggccgtcg
      121 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      181 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      241 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
      301 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
      361 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
      421 cggcatccgc ttacagacaa gctgtgacct tctccgggag ctgcatgtgt cagaggtttt
      481 caccgtcatc accgaaacgc gcgagacaaa agggcctcgt gatacgccta tttttatagg
      541 ttaatgtcat gagcggcgtt gtgacaattt accgaacaac tccgcggccg ggaagccgat
      601 ctcggcttga acgaattgtt aggtggcggt acttgggtcg atatcaaagt gcatcacttc
      661 ttcccgtatg cccaactttg tatagagagc cactgcggga tcgtcaccgt aatctgcttg
      721 cacgtagatc acataagcac caagcgcgtt ggcctcatgc ttgaggagat tgatgagcgc
      781 ggtggcaatg ccctgcctcc ggtgctctcc ggagactgcg agatcataga tatagatctc
      841 actacgcggc tgctcaaact tgggcagaac gtaagccgcg agagcgccaa caaccgcttc
      901 ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg agca
//
