LOCUS       sequence-87606-e        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI E-F nos-T c2
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      43..295
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(366..382)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(366..382)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(379..401)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    390..406
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(414..420,421..438,439..444))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    459..480
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(597..614)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(848..867)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agctcggtac ctcgcgaatg catctagatg gggtctctaa tcgatcgttc aaacatttgg
       61 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
      121 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
      181 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
      241 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatctgagt
      301 gagaccccat cggatcccgg gcccgtcgac tgcagaggcc tgcatgcaag cttggcgtaa
      361 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      421 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      481 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      541 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
      601 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      661 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      721 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      781 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      841 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      901 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      961 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1021 gtgcacgaac cccccgttca gcccgaccgc tgc
//
