LOCUS       sequence-87607-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI E-F nos-T c2
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(57..309)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(359..376)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(359..375)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(368..390)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(584..603)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     703..725
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(763..781)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcttgcatgc aggcctctgc agtcgacggg cccgggatcc gatggggtct cactcagatc
       61 tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt
      121 ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa
      181 taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca gaaattatat
      241 gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt
      301 tgaacgatcg attagagacc ccatctagat gcattcgcga ggtaccgagc tcgaattcac
      361 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      421 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
      481 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
      541 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
      601 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
      661 gtctgctccc ggcatccgct tacagacaag ctgtgacctt ctccgggagc tgcatgtgtc
      721 agaggttttc accgtcatca ccgaaacgcg cgagacaaaa gggcctcgtg atacgcctat
      781 ttttataggt taatgtcatg agcggcgttg tgacaattta ccgaacaact ccgcggccgg
      841 gaagccgatc tcggcttgaa cgaattgtta ggtggcggta cttgggtcga tatcaaagtg
      901 catcacttct tcccgtatgc ccaactttgt atagagagcc actgcggg
//
