LOCUS       sequence-87610-e        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI A-C 35S c2
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        500..845
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     788..808
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(932..948)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(932..948)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(945..967)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    956..972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(980..986,987..1004,1005..1010))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 tggtctctgc ggagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta
       61 gagaggctta cgcagcacga ctcatcaaga cgatctaccc gagcaataat ctccaggaaa
      121 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
      181 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
      241 aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc
      301 ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg
      361 taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct
      421 tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct
      481 cagaagagca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg
      541 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
      601 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
      661 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa cacgttccaa
      721 ccacgacttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      781 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      841 gaacacgcac ctgagacctc gaatgcatct agatatcgga tcccgggccc gtcgactgca
      901 gaggcctgca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      961 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     1021 cctaatgagt ga
//
