LOCUS       sequence-87616-e        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI B-C mOrange
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..786
                     /codon_start=1
                     /product="orange monomeric derivative of DsRed fluorescent 
                     protein (Shanet et al., 2004)"
                     /label=mOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(876..892)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(876..892)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(889..911)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    900..916
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(924..930,931..948,949..954))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    969..990
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 agctcggtac ctcgcgaatg catctagata tcggatcccg ggcccgtcga ctgcagaggt
       61 acgggtctca tctgaacaat ggtgagcaag ggcgaggaga ataacatggc catcatcaag
      121 gagttcatgc gcttcaaggt gcgcatggag ggctctgtga acggccacga gttcgagatc
      181 gagggcgagg gcgagggccg cccctacgag ggctttcaga ccgctaagct gaaggtgacc
      241 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcac ctacggctcc
      301 aaggcctacg tgaagcaccc cgccgacatc cccgactact tcaagctgtc cttccccgag
      361 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      421 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
      481 ccctccgacg gccccgtaat gcagaagaaa accatgggct gggaggcctc ctccgagcgg
      541 atgtaccccg aggacggcgc cctgaagggc gagatcaaga tgaggctgaa gctgaaggac
      601 ggcggccact acacctccga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      661 cccggcgcct acatcgtcgg catcaagttg gacatcacct cccacaacga ggactacacc
      721 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
      781 tacaagggtg gaggaggttc tggaggcggt ggaagtggtg gcggaggtag cggcacctga
      841 gacctctgcc tgcatgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
      901 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      961 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     1021 tcgggaaacc tgtc
//
