LOCUS       sequence-87623-e        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI C-D mCherry noATG
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..726)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     566..584
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(824..840)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(824..840)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(837..859)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    848..864
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(872..878,879..896,897..902))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    917..938
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 agacaacaga ggtctcacct tcttgtacag ctcgtccatg ccgccggtgg agtggcggcc
       61 ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa
      121 cttgatgttg acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt
      181 cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc
      241 cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtttt
      301 cttctgcatt acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat
      361 gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa
      421 gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat
      481 gtcggcgggg tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc
      541 ccaggcgaag ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc
      601 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc
      661 catgtgcacc ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt
      721 gctcacggtg tgagacccgt aaagtcttca tcgaatgcat ctagatatcg gatcccgggc
      781 ccgtcgactg cagaggcctg catgcaagct tggcgtaatc atggtcatag ctgtttcctg
      841 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      901 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      961 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
//
