LOCUS       sequence-87632-e        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI C-D GFP c1
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(72..74,75..77,78..788)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(117..138)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(378..397)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     725..746
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(831..847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(831..847)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(844..866)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    855..871
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(879..885,886..903,904..909))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    924..945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tcggtacctc gcgaatgcat ctagatatcg gatcccgggc ccgtcgactg cagaggtacg
       61 ggtctcacac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      121 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      181 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
      241 cctggcccac cctcgtgacc accttcacct acggcgtgca gtgcttcagc cgctaccccg
      301 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
      361 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
      421 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
      481 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
      541 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
      601 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
      661 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
      721 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactcacggc atggacgagc
      781 tgtacaagaa ggtgagacct ctgcctgcat gcaagcttgg cgtaatcatg gtcatagctg
      841 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      901 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      961 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     1021 gcggggagag gcg
//
