LOCUS       sequence-8764-ex        9901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Guo XV, Monteleone M, Klotzsche M, Kamionka A, Hillen W, Braunstein 
            M, Ehrt S, Schnappinger D
  TITLE     Silencing Mycobacterium smegmatis by using tetracycline repressors.
  JOURNAL   J Bacteriol. 2007 Jul . 189(13):4614-23.
  PUBMED    17483222
REFERENCE   2  (bases 1 to 9901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 2007 Jul . 189(13):4614-23."
COMMENT     Sequence Label: pME1mL1
FEATURES             Location/Qualifiers
     source          1..9901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      20..47
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             413..3460
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(415..437)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    3724..3858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3761..3780)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3815..3834
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3931..3947)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3932..3948)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      6772..6799
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             6984..7607
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(7057..7076)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     rep_origin      9073..9661
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9562..9581
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9815..9832
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 actagttgat tagctaagca gaaggccatc ctgacggatg gcctttttgc gtttaatact
       61 gtttaaactc tagaaatatt ggatcgtcgg caccgtcacg gccgtgggag gcggcacgat
      121 ccgcgacgtg atgatcggcc gcatccccac ggtgctgcgc agtgagctct acgccatccc
      181 ggcgttgatc tgtgcgttcg cacgcacagg cccggtgtga gaagggtctc tgacgagcgg
      241 gagaactccc tatcagtgat agagtttgtc ctccctatca gtgatagata ggctctggga
      301 gtacccgtgt gtacgaccag cacggcatac atcatttcga cgccgagaga ttcgccgccc
      361 gaaatgagca cgatccgcat gcgaaggaga tatacatatg gagatcgatc ccgtcgtttt
      421 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
      481 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
      541 gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga
      601 aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca
      661 gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta cggtcaatcc
      721 gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga
      781 aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact cggcgtttca
      841 tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc cgtctgaatt
      901 tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg tgctgcgttg
      961 gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca ttttccgtga
     1021 cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg ccactcgctt
     1081 taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg
     1141 tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg ccagcggcac
     1201 cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc gcgtcacact
     1261 acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc
     1321 ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg
     1381 tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc cgttgctgat
     1441 tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg atgagcagac
     1501 gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc gctgttcgca
     1561 ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga
     1621 tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg atgatccgcg
     1681 ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc
     1741 gagtgtgatc atctggtcgc tggggaatga atcaggccac ggcgctaatc acgacgcgct
     1801 gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag gcggcggagc
     1861 cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc
     1921 cttcccggct gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg gagagacgcg
     1981 cccgctgatc ctttgcgaat acgcccacgc gatgggtaac agtcttggcg gtttcgctaa
     2041 atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct gggactgggt
     2101 ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggtcggctt acggcggtga
     2161 ttttggcgat acgccgaacg atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg
     2221 cacgccgcat ccagcgctga cggaagcaaa acaccagcag cagtttttcc agttccgttt
     2281 atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg ataacgagct
     2341 cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag tgcctctgga
     2401 tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc cggagagcgc
     2461 cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc
     2521 cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg tgacgctccc
     2581 cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt gcatcgagct
     2641 gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg
     2701 cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac cgctggataa
     2761 cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg aacgctggaa
     2821 ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag atacacttgc
     2881 tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa ccttatttat
     2941 cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg ttgatgttga
     3001 agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc tggcgcaggt
     3061 agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg accgccttac
     3121 tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc cgtacgtctt
     3181 cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc cacaccagtg
     3241 gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga tggaaaccag
     3301 ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg gtttccatat
     3361 ggggattggt ggcgacgact cctggagccc gtcagtatcg gcggaattcc agctgagcgc
     3421 cggtcgctac cattaccagt tggtctggtg tcaaaaataa taataaccgg gcaggccatg
     3481 tctgcccgta tttcgcgtaa ggaaatccat tatgtactat ttaaaaaaca caaacttttg
     3541 gatgttcggt ttattctttt tcttttactt ttttatcatg ggagcctact tcccgttttt
     3601 cccgatttgg ctacatgaca tcaaccatat cagcaaaagt gatacgggta ttatttttgc
     3661 cgctatttct ctgttctcgc tattattcca accgctgttt ggtctgcttt ctgacaaact
     3721 cggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     3781 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3841 tcttatcatg tctggatcct ctagagtcga cctgcagcca agctttagct aattaattgg
     3901 ggaccctaga ggtccccttt tttattttat cgataccgtc gacctcgagg gggggcccgc
     3961 cggggatcat cgagccgaga acgttatcga agttggtcat gtgtaatccc ctcgtttgaa
     4021 ctttggatta agcgtagata cacccttgga caagccagtt ggattcggag acaagcaaat
     4081 tcagccttaa aaagggcgag gcctgcggtg gtggaacacc gcagggcctc taaccgctcg
     4141 acgcgctgca ccaaccagcc cgcgaacggc tggcagccag cgtaaggcgc ggctcatcgg
     4201 gcggcgttcg ccacgatgtc ctgcacttcg agccaagcct cgaacacctg ctggtgtgca
     4261 cgactcaccc ggttgttgac accgcgcgcg gccgtgcggg ctcggtgggg cggctgtgtc
     4321 gcccttgcca gcgtgagtag cgcgtacctc acctcgccca acaggtcgca cacagccgat
     4381 tcgtacgcca taaagccagg tgagcccacc agctccgtaa gttcgggcgc tgtgtggctc
     4441 gtacccgcgc attcaggcgg cagggggtct aacgggtcta aggcggcgtg tacggccgcc
     4501 acagcggctc tcagcggccc ggaaacgtcc tcgaaacgac gcatgtgttc ctcctggttg
     4561 gtacaggtgg ttgggggtgc tcggctgtcg ctggtgttcc accaccaggg ctcgacggga
     4621 gagcggggga gtgtgcagtt gtggggtggc ccctcagcga aatatctgac ttggagctcg
     4681 tgtcggacca tacaccggtg attaatcgtg gtctactacc aagcgtgagc cacgtcgccg
     4741 acgaatttga gcagctctgg ctgccgtact ggccgctggc aagcgacgat ctgctcgagg
     4801 ggatctaccg ccaaagccgc gcgtcggccc taggccgccg gtacatcgag gcgaacccaa
     4861 cagcgctggc aaacctgctg gtcgtggacg tagaccatcc agacgcagcg ctccgagcgc
     4921 tcagcgcccg ggggtcccat ccgctgccca acgcgatcgt gggcaatcgc gccaacggcc
     4981 acgcacacgc agtgtgggca ctcaacgccc ctgttccacg caccgaatac gcgcggcgta
     5041 agccgctcgc atacatggcg gcgtgcgccg aaggccttcg gcgcgccgtc gacggcgacc
     5101 gcagttactc aggcctcatg accaaaaacc ccggccacat cgcctgggaa acggaatggc
     5161 tccactcaga tctctacaca ctcagccaca tcgaggccga gctcggcgcg aacatgccac
     5221 cgccgcgctg gcgtcagcag accacgtaca aagcggctcc gacgccgcta gggcggaatt
     5281 gcgcactgtt cgattccgtc aggttgtggg cctatcgtcc cgccctcatg cggatctacc
     5341 tgccgacccg gaacgtggac ggactcggcc gcgcgatcta tgccgagtgc cacgcgcgaa
     5401 acgccgaatt cccgtgcaac gacgtgtgtc ccggaccgct accggacagc gaggtccgcg
     5461 ccatcgccaa cagcatttgg cgttggatca caaccaagtc gcgcatttgg gcggacggga
     5521 tcgtggtcta cgaggccaca ctcagtgcgc gccagtcggc catctcgcgg aagggcgcag
     5581 cagcgcgcac ggcggcgagc acagttgcgc ggcgcgcaaa gtccgcgtca gccatggagg
     5641 cattgctatg agcgacggct acagcgacgg ctacagcgac ggctacaacc ggcagccgac
     5701 tgtccgcaaa aagcggcgcg tgaccgccgc cgaaggcgct cgaatcaccg gactatccga
     5761 acgccacgtc gtccggctcg tggcgcagga acgcagcgag tggctcgccg agcaggctgc
     5821 acgccgcgaa cgcatccgcg cctatcacga cgacgagggc cactcttggc cgcaaacggc
     5881 caaacatttc gggctgcatc tggacaccgt taagcgactc ggctatcggg cgaggaaaga
     5941 gcgtgcggca gaacaggaag cggctcaaaa ggcccacaac gaagccgaca atccaccgct
     6001 gttctaacgc aattggggag cgggtgtcgc gggggttccg tggggggttc cgttgcaacg
     6061 ggtcggacag gtaaaagtcc tggtagacgc tagttttctg gtttgggcca tgcctgtctc
     6121 gttgcgtgtt tcgttgcgcc cgttttgaat accagccaga cgagacgggg ttctacgaat
     6181 cttggtcgat accaagccat ttccgctgaa tatcggggag ctcaccgcca gaatcggtgg
     6241 ttgtggtgat gtacgtggcg aactccgttg tagtgcctgt ggtggcatcc gtggccactc
     6301 tcgttgcacg gttcgttgtg ccgttacagg ccccgttgac agctcaccga acgtagttaa
     6361 aacatgctgg tcaaactagg tttaccaacg atacgagtca gctcatctag ggccagttct
     6421 aggcgttgtt cgttgcgcgg ttcgttgcgc atgtttcgtg tggttgctag atggctccgc
     6481 aaccacacgc ttcgaggttg agtgcttcca gcacgggcgc gatccagaag aacttcgtcg
     6541 tgcgactgtc ctcgttgatc cttgccgagc tgggatggaa gctcggccga ccaccctgga
     6601 ggagatgatc gaggatgcca gggcctttca cgcccgccgc tgctgagcgt ccgccgccgg
     6661 gcccgcaccg ccgtcggccg gcccgctccg ggctcgcagc agcgggcttc ggcgcgggcc
     6721 cggggctccc gagcgcgggc ggggctccgg gcggccgctg attagctaag cagaaggcca
     6781 tcctgacgga tggccttttt gcgtttaata ctgcatgcac tctagaaata ttggatcgtc
     6841 gcaccgggtt aagcctggag tgcggtggtg cctggtcggc attttcgcag tcgagggctc
     6901 tcgtgtagcc tgggcgagtt gccgacgcag gcgaccctcn tgccacggat ccgcatggta
     6961 ccatcaggag gaatcacttc gcaatgtcta gattagataa aagtaaagtg attaacagcg
     7021 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga
     7081 agcttggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg gccctgctcg
     7141 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttagaagggg
     7201 aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc
     7261 atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc
     7321 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaac gcgttatatg
     7381 cactcagcgc tgtggggcat tttaccttag gttgcgtatt ggaagatcaa gagcatcaag
     7441 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag
     7501 ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga
     7561 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtcttaatga ccatgggcta
     7621 gcggctttag ctaattaatt ggggacccta gaggtcccct tttttatttt gcggccgccg
     7681 ggggccgggg gcggcgccgg gcggcccggg gcgtcaggcg ccgggggcgg tgtccggcgg
     7741 cccccagagg aactgcgcca gttcctccgg atcggtgaag ccggagagat ccagcggggt
     7801 ctcctcgaac acctcgaagt cgtgcaggaa ggtgaaggcg agcagttcgc gggcgaagtc
     7861 ctcggtccgc ttccactgcg ccccgtcgag cagcgcggcc aggatctcgc ggtcgccccg
     7921 gaaggcgttg agatgcagtt gcaccaggct gtagcgggag tctcccgcat agacgtcggt
     7981 gaagtcgacg atcccggtga cctcggtcgc ggccaggtcc acgaagatgt tggtcccgtg
     8041 caggtcgccg tggacgaacc ggggttcgcg gccggccagc agcgtgtcca cgtccggcag
     8101 ccagtcctcc aggcggtcca gcagccgggg cgagaggtag ccccacccgc ggtggtcctc
     8161 gacggtcgcc gcgcggcgtt cccgcagcag ttccgggaag acctcggaat ggggggtgag
     8221 cacggtgttc ccggtcagcg gcaccctgtg cagccggccg agcacccggc cgagttcgcg
     8281 ggccagggcg agcagcgcgt tccggtcggt cgtgccgtcc atcgcggacc gccaggtggt
     8341 gccggtcatc cggctcatca ccaggtaggg ccacggccag gctccggtgc cgggccgcag
     8401 ctcgccgcgg ccgaggaggc ggggcaccgg caccggggcg tccgccagga ccgcgtacgc
     8461 ctccgactcc gacgcgaggc tctccggacc gcaccagtgc tcgccgaaca gcttgatcac
     8521 cgggtcgggc tcgccgacca gtacggggtt ggtgctctcg ccgggcaccc gcagcaccgg
     8581 cggcaccggc agcccgagct cctccagggc tcggcgggcc agcggctccc agaattcctg
     8641 gtcgttccgc aggctcgcgt aggaatcatc cgaatcaata cggtcgagaa gtaacaggga
     8701 ttcttgtgtc acagcggacc tctattcaca gggtacgggc cggcttaatt ccgcacggcc
     8761 ggtcgcgaca cggcctgtcc gcaccgcggt caggcgttga cgatgacggg ctggtcggcc
     8821 acgtccgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     8881 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8941 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     9001 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     9061 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     9121 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     9181 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     9241 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     9301 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     9361 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     9421 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     9481 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     9541 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     9601 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     9661 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     9721 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     9781 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     9841 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcta
     9901 g
//
