LOCUS       sequence-87650-e        1091 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 
            (498–1344 with S311DT315D) from blasticidin resistance retroviral 
            vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pWZLblasti-HA-MEK1-MEK5DD
FEATURES             Location/Qualifiers
     source          1..1091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             68..94
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tcctcccttt atccagccct cactccttct ctaggcgccg gccggatccg atatcgccgc
       61 caccatgtac ccatacgatg ttccagatta cgctcccaag aagaagccga cgcccatcca
      121 gctgaacccg gcccccgacg gctctgcagt taacgggacc agctctgcgg agaccaactt
      181 ggaggccttg cagaagaagc tggaggagct agagcttgat gagcagcagc gaaagcgcct
      241 tgaggccttt cttacccaga agcagaaggt gggagaactg aaggatgacg acatacgata
      301 tcgggacact cttggtcatg gcaacggagg cacagtctac aaagcatatc atgtcccgag
      361 tgggaaaata ttagctgtaa aggtcatact actagatatt acactggaac ttcagaagca
      421 aattatgtct gaattggaaa ttctttataa gtgcgattca tcatatatca ttggatttta
      481 tggagcattt tttgtagaaa acaggatttc aatatgtaca gaattcatgg atgggggatc
      541 tttggatgta tataggaaaa tgccagaaca tgtccttgga agaattgcag tagcagttgt
      601 taaaggcctt acttatttgt ggagtttaaa gattttacat agagacgtga agccctccaa
      661 tatgctagta aacacaagag gacaggttaa gctgtgtgat tttggagtta gcactcagct
      721 ggtgaatgat atagccaagg actatgttgg aacaaatgct tatatggcgc ctgaaaggat
      781 ttcaggggag cagtatggaa ttcattctga tgtctggagc ttaggaatct cttttatgga
      841 gcttgctctt gggaggtttc catatcctca gattcagaaa aaccagggat ctttaatgcc
      901 tctccagctt ctgcagtgca ttgttgatga ggattcgccc gtccttccag ttggagagtt
      961 ctcggagcca tttgtacatt tcatcactca gtgtatgcga aaacagccaa aagaaaggcc
     1021 agcacctgaa gaattgatgg gccacccgtt catcgtgcag ttcaatgatg gaaatgccgc
     1081 cgtggtgtcc a
//
