LOCUS       sequence-87651-e        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI D-E mCherry + link c1
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             122..829
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(264..282)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(872..888)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(872..888)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(885..907)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    896..912
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(920..926,927..944,945..950))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    965..986
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 agctcggtac ctcgcgaatg catctagata tcggatcccg ggcccgtcga ctgcagaggt
       61 acgggtctca aagggaggtg gaggaggttc tggaggcggt ggaagtggtg gcggaggtag
      121 cgtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
      181 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
      241 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
      301 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
      361 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
      421 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
      481 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
      541 gcagaagaaa accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
      601 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
      661 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
      721 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
      781 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaaa atctgagacc
      841 tctgcctgca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      901 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      961 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1021 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcgggga
//
