LOCUS       sequence-87653-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 
            (498–1344 with S311DT315D) from blasticidin resistance retroviral 
            vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pWZLblasti-HA-MEK1-MEK5DD
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tggtggaaaa taaccggaat tggtcgactc acgggggccc ctgctgctgg ctccgcctct
       61 cctccagcgc ccggcacacc cacatggaca ccacggcggc atttccatca ttgaactgca
      121 cgatgaacgg gtggcccatc aattcttcag gtgctggcct ttcttttggc tgttttcgca
      181 tacactgagt gatgaaatgt acaaatggct ccgagaactc tccaactgga aggacgggcg
      241 aatcctcatc aacaatgcac tgcagaagct ggagaggcat taaagatccc tggtttttct
      301 gaatctgagg atatggaaac ctcccaagag caagctccat aaaagagatt cctaagctcc
      361 agacatcaga atgaattcca tactgctccc ctgaaatcct ttcaggcgcc atataagcat
      421 ttgttccaac atagtccttg gctatatcat tcaccagctg agtgctaact ccaaaatcac
      481 acagcttaac ctgtcctctt gtgtttacta gcatattgga gggcttcacg tctctatgta
      541 aaatctttaa actccacaaa taagtaaggc ctttaacaac tgctactgca attcttccaa
      601 ggacatgttc tggcattttc ctatatacat ccaaagatcc cccatccatg aattctgtac
      661 atattgaaat cctgttttct acaaaaaatg ctccataaaa tccaatgata tatgatgaat
      721 cgcacttata aagaatttcc aattcagaca taatttgctt ctgaagttcc agtgtaatat
      781 ctagtagtat gacctttaca gctaatattt tcccactcgg gacatgatat gctttgtaga
      841 ctgtgcctcc gttgccatga ccaagagtgt cccgatatcg tatgtcgtca tccttcagt
//
