LOCUS       sequence-87658-e        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI C-D mOrange noATG
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(85..789)
                     /codon_start=1
                     /product="orange monomeric derivative of DsRed fluorescent 
                     protein (Shanet et al., 2004)"
                     /label=mOrange
                     /note="mammalian codon-optimized"
                     /translation="VSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGF
                     QTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(836..853)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(836..852)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(845..867)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agcttgcatg caggcctctg cagtcgacgg gcccgggatc cgatatctag atgcattcgt
       61 agaagacaac agaggtctca ccttcttgta cagctcgtcc atgccgccgg tggagtggcg
      121 gccctcggcg cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc
      181 caacttgatg ccgacgatgt aggcgccggg cagctgcacg ggcttcttgg ccttgtaggt
      241 ggtcttgacc tcggaggtgt agtggccgcc gtccttcagc ttcagcctca tcttgatctc
      301 gcccttcagg gcgccgtcct cggggtacat ccgctcggag gaggcctccc agcccatggt
      361 tttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta
      421 gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc
      481 gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttga agtagtcggg
      541 gatgtcggcg gggtgcttca cgtaggcctt ggagccgtag gtgaactgag gggacaggat
      601 gtcccaggcg aagggcaggg ggccaccctt ggtcaccttc agcttagcgg tctgaaagcc
      661 ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacagagcc
      721 ctccatgcgc accttgaagc gcatgaactc cttgatgatg gccatgttat tctcctcgcc
      781 cttgctcacg gtgtgagacc cgtaaagtct tcatcgaggt accgagctcg aattcactgg
      841 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      901 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      961 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
     1021 atctgtgcgg tatttcacac cgcatatgg
//
