LOCUS       sequence-8766-ex        6287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Guo XV, Monteleone M, Klotzsche M, Kamionka A, Hillen W, Braunstein 
            M, Ehrt S, Schnappinger D
  TITLE     Silencing Mycobacterium smegmatis by using tetracycline repressors.
  JOURNAL   J Bacteriol. 2007 Jul . 189(13):4614-23.
  PUBMED    17483222
REFERENCE   2  (bases 1 to 6287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 2007 Jul . 189(13):4614-23."
COMMENT     Sequence Label: pTE-4S-0X
FEATURES             Location/Qualifiers
     source          1..6287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      14..41
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(523..542)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(1226..1242)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1227..1243)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     rep_origin      5452..6040
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5941..5960
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6194..6211
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgattagcta agcagaaggc catcctgacg gatggccttt ttgcgtttaa tactgtttgc
       61 ggccgcgttt aaacctgcag gcacgtggca tgcctcgagg atatcagatc tactagaaat
      121 attggatcgt cggcaccgtc acggccgtgg gaggcggcac gatccgcgac gtgatgatcg
      181 gccgcatccc cacggtgctg cgcagtgagc tctacgccat cccggcgttg atctgtgcgt
      241 tcgcacgcac aggcccggtg tgagaagggt ctctgacgag cgggagaacc cacccggggt
      301 gggcgagttt gtcctgcgtg tgctcggtcg agtaggctct gggagtaccc gtgtgtacga
      361 ccagcacggc atacatcatt tcgacgccga gagattcgcc gcccgaaatg agcacgatcc
      421 gcggtaccat caggaggaat cacttcgcaa tgtctagatt agataaaagt aaagtgatta
      481 acagcgcatt ggcgctgggt aatgaggtcg gaatcgaagg tgtaacaacc cgtaaactcg
      541 cccagaagct tggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggccc
      601 tactggatgc gctggcggtg gagatcttgg cgcgtcatca tgattattca ctgcctgcgg
      661 cgggggaatc ctggcagtca tttctgcgca ataatgcaat gagtttccgc cgcgcgctgc
      721 tgcgttaccg tgacggggca aaagtgcacc tcggcacgcg tcctgatgaa aaacagtatg
      781 atacggtgga aacccagtta cgctttatga cagaaaacgg cttttcactg cgcgacgggt
      841 tatatgcgat ttcagcggtc agtcatttta ccttaggtgc cgtactggag cagcaggagc
      901 atactgccgc cctgaccgac cgccctgcag caccggacga aaacctgccg ccgctattgc
      961 gggaagcgct gcagattatg gacagtgatg atggtgagca ggcctttctg catggcctgg
     1021 agagcctgat ccgggggttt gaggtgcagc ttacggcact gttgcaaata gtctgaggcc
     1081 atggtgacct catgactcgc tagccagctg gggaccctag aggtcccctt ngaaaaaatt
     1141 tttaaaataa aaaaggggac ctctagggtc cccaattaaa tagttaacac tagtatttaa
     1201 atcaattgat gcatattaat taaatcgata ccgtcgacct cgaggggggg cccgccgggg
     1261 atcatcgagc cgagaacgtt atcgaagttg gtcatgtgta atcccctcgt ttgaactttg
     1321 gattaagcgt agatacaccc ttggacaagc cagttggatt cggagacaag caaattcagc
     1381 cttaaaaagg gcgaggcctg cggtggtgga acaccgcagg gcctctaacc gctcgacgcg
     1441 ctgcaccaac cagcccgcga acggctggca gccagcgtaa ggcgcggctc atcgggcggc
     1501 gttcgccacg atgtcctgca cttcgagcca agcctcgaac acctgctggt gtgcacgact
     1561 cacccggttg ttgacaccgc gcgcggccgt gcgggctcgg tggggcggct gtgtcgccct
     1621 tgccagcgtg agtagcgcgt acctcacctc gcccaacagg tcgcacacag ccgattcgta
     1681 cgccataaag ccaggtgagc ccaccagctc cgtaagttcg ggcgctgtgt ggctcgtacc
     1741 cgcgcattca ggcggcaggg ggtctaacgg gtctaaggcg gcgtgtacgg ccgccacagc
     1801 ggctctcagc ggcccggaaa cgtcctcgaa acgacgcatg tgttcctcct ggttggtaca
     1861 ggtggttggg ggtgctcggc tgtcgctggt gttccaccac cagggctcga cgggagagcg
     1921 ggggagtgtg cagttgtggg gtggcccctc agcgaaatat ctgacttgga gctcgtgtcg
     1981 gaccatacac cggtgattaa tcgtggtcta ctaccaagcg tgagccacgt cgccgacgaa
     2041 tttgagcagc tctggctgcc gtactggccg ctggcaagcg acgatctgct cgaggggatc
     2101 taccgccaaa gccgcgcgtc ggccctaggc cgccggtaca tcgaggcgaa cccaacagcg
     2161 ctggcaaacc tgctggtcgt ggacgtagac catccagacg cagcgctccg agcgctcagc
     2221 gcccgggggt cccatccgct gcccaacgcg atcgtgggca atcgcgccaa cggccacgca
     2281 cacgcagtgt gggcactcaa cgcccctgtt ccacgcaccg aatacgcgcg gcgtaagccg
     2341 ctcgcataca tggcggcgtg cgccgaaggc cttcggcgcg ccgtcgacgg cgaccgcagt
     2401 tactcaggcc tcatgaccaa aaaccccggc cacatcgcct gggaaacgga atggctccac
     2461 tcagatctct acacactcag ccacatcgag gccgagctcg gcgcgaacat gccaccgccg
     2521 cgctggcgtc agcagaccac gtacaaagcg gctccgacgc cgctagggcg gaattgcgca
     2581 ctgttcgatt ccgtcaggtt gtgggcctat cgtcccgccc tcatgcggat ctacctgccg
     2641 acccggaacg tggacggact cggccgcgcg atctatgccg agtgccacgc gcgaaacgcc
     2701 gaattcccgt gcaacgacgt gtgtcccgga ccgctaccgg acagcgaggt ccgcgccatc
     2761 gccaacagca tttggcgttg gatcacaacc aagtcgcgca tttgggcgga cgggatcgtg
     2821 gtctacgagg ccacactcag tgcgcgccag tcggccatct cgcggaaggg cgcagcagcg
     2881 cgcacggcgg cgagcacagt tgcgcggcgc gcaaagtccg cgtcagccat ggaggcattg
     2941 ctatgagcga cggctacagc gacggctaca gcgacggcta caaccggcag ccgactgtcc
     3001 gcaaaaagcg gcgcgtgacc gccgccgaag gcgctcgaat caccggacta tccgaacgcc
     3061 acgtcgtccg gctcgtggcg caggaacgca gcgagtggct cgccgagcag gctgcacgcc
     3121 gcgaacgcat ccgcgcctat cacgacgacg agggccactc ttggccgcaa acggccaaac
     3181 atttcgggct gcatctggac accgttaagc gactcggcta tcgggcgagg aaagagcgtg
     3241 cggcagaaca ggaagcggct caaaaggccc acaacgaagc cgacaatcca ccgctgttct
     3301 aacgcaattg gggagcgggt gtcgcggggg ttccgtgggg ggttccgttg caacgggtcg
     3361 gacaggtaaa agtcctggta gacgctagtt ttctggtttg ggccatgcct gtctcgttgc
     3421 gtgtttcgtt gcgcccgttt tgaataccag ccagacgaga cggggttcta cgaatcttgg
     3481 tcgataccaa gccatttccg ctgaatatcg gggagctcac cgccagaatc ggtggttgtg
     3541 gtgatgtacg tggcgaactc cgttgtagtg cctgtggtgg catccgtggc cactctcgtt
     3601 gcacggttcg ttgtgccgtt acaggccccg ttgacagctc accgaacgta gttaaaacat
     3661 gctggtcaaa ctaggtttac caacgatacg agtcagctca tctagggcca gttctaggcg
     3721 ttgttcgttg cgcggttcgt tgcgcatgtt tcgtgtggtt gctagatggc tccgcaacca
     3781 cacgcttcga ggttgagtgc ttccagcacg ggcgcgatcc agaagaactt cgtcgtgcga
     3841 ctgtcctcgt tgatccttgc cgagctggga tggaagctcg gccgaccacc ctggaggaga
     3901 tgatcgagga tgccagggcc tttcacgccc gccgctgctg agcgtccgcc gccgggcccg
     3961 caccgccgtc ggccggcccg ctccgggctc gcagcagcgg gcttcggcgc gggcccgggg
     4021 ctcccgagcg cgggcggggc tccgggcggc cggccgccgg gggccggggg cggcgccggg
     4081 cggcccgggg cgtcaggcgc cgggggcggt gtccggcggc ccccagagga actgcgccag
     4141 ttcctccgga tcggtgaagc cggagagatc cagcggggtc tcctcgaaca cctcgaagtc
     4201 gtgcaggaag gtgaaggcga gcagttcgcg ggcgaagtcc tcggtccgct tccactgcgc
     4261 cccgtcgagc agcgcggcca ggatctcgcg gtcgccccgg aaggcgttga gatgcagttg
     4321 caccaggctg tagcgggagt ctcccgcata gacgtcggtg aagtcgacga tcccggtgac
     4381 ctcggtcgcg gccaggtcca cgaagatgtt ggtcccgtgc aggtcgccgt ggacgaaccg
     4441 gggttcgcgg ccggccagca gcgtgtccac gtccggcagc cagtcctcca ggcggtccag
     4501 cagccggggc gagaggtagc cccacccgcg gtggtcctcg acggtcgccg cgcggcgttc
     4561 ccgcagcagt tccgggaaga cctcggaatg gggggtgagc acggtgttcc cggtcagcgg
     4621 caccctgtgc agccggccga gcacccggcc gagttcgcgg gccagggcga gcagcgcgtt
     4681 ccggtcggtc gtgccgtcca tcgcggaccg ccaggtggtg ccggtcatcc ggctcatcac
     4741 caggtagggc cacggccagg ctccggtgcc gggccgcagc tcgccgcggc cgaggaggcg
     4801 gggcaccggc accggggcgt ccgccaggac cgcgtacgcc tccgactccg acgcgaggct
     4861 ctccggaccg caccagtgct cgccgaacag cttgatcacc gggtcgggct cgccgaccag
     4921 tacggggttg gtgctctcgc cgggcacccg cagcaccggc ggcaccggca gcccgagctc
     4981 ctccagggct cggcgggcca gcggctccca gaattcctgg tcgttccgca ggctcgcgta
     5041 ggaatcatcc gaatcaatac ggtcgagaag taacagggat tcttgtgtca cagcggacct
     5101 ctattcacag ggtacgggcc ggcttaattc cgcacggccg gtcgcgacac ggcctgtccg
     5161 caccgcggtc aggcgttgac gatgacgggc tggtcggcca cgtccgggga gtcaggcaac
     5221 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     5281 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     5341 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5401 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5461 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5521 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5581 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5641 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5701 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5761 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5821 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5881 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5941 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6001 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6061 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     6121 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     6181 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     6241 gcctctcccc gcgcgttggc cgattcatta atgcagctag aactagt
//
