LOCUS       sequence-87665-e        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: pUC57 (Gent)
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(87..103)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(87..103)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(100..122)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    111..127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(135..141,142..159,160..165))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..201
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(318..335)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(569..588)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agctcggtac ctcgcgaatg catctagata tcggatcccg ggcccgtcga ctgcagaggc
       61 ctgcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      121 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      181 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      241 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      301 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      361 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      421 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      481 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      541 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      601 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      661 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      721 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      781 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      841 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      901 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
      961 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
//
