LOCUS       sequence-87667-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: pUC57 (Gent)
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(81..98)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(81..97)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(90..112)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(306..325)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     425..447
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(485..503)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agcttgcatg caggcctctg cagtcgacgg gcccgggatc cgatatctag atgcattcgc
       61 gaggtaccga gctcgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
      121 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      181 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
      241 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
      301 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
      361 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
      421 ttctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacaa
      481 aagggcctcg tgatacgcct atttttatag gttaatgtca tgagcggcgt tgtgacaatt
      541 taccgaacaa ctccgcggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg
      601 tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag
      661 ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt
      721 tggcctcatg cttgaggaga ttgatgagcg cggtggcaat gccctgcctc cggtgctctc
      781 cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa
      841 cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg
      901 tcttactacg gagcaagttc ccgaggtaat cgga
//
