LOCUS       sequence-87671-e        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI D-E T-Saph + linker c1
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(123..125,126..836)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVMVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(165..186)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(426..445)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     773..794
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(882..898)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(882..898)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(895..917)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    906..922
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(930..936,937..954,955..960))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    975..996
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gagctcggta cctcgcgaat gcatctagat atcggatccc gggcccgtcg actgcagagg
       61 tacgggtctc aaagggaggt ggaggaggtt ctggaggcgg tggaagtggt ggcggaggta
      121 gcgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      181 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      241 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      301 tcgtgaccac cttctcctac ggcgtgatgg tgttcgcccg ctaccccgac cacatgaagc
      361 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
      421 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      481 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
      541 agctggagta caacttcaac agccacaacg tctatatcat ggccgacaag cagaagaacg
      601 gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgccg
      661 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
      721 acctgagcat ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
      781 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa
      841 atctgagacc tctgcctgca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg
      901 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      961 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1021 ttccagt
//
