LOCUS       sequence-87677-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli 
            sigma-70 region 4. Used as two-hybrid control. For cloning purposes,
            see Addgene plasmid 53734..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Dove SL, Joung JK, Hochschild A
  TITLE     Activation of prokaryotic transcription through arbitrary 
            protein-protein contacts.
  JOURNAL   Nature. 1997 Apr 10;386(6625):627-30.
  PUBMED    9121589
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "1997-04-10"; volume: "386(6625)"; pages: "627-30"
COMMENT     Sequence Label: pBR-alpha-sigma-70 D581G
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggccacgatg cgtccggcgt agaggatcct aggttaatcg tccaggaagc tacgcagcac
       61 ttcagaacgg ctcgggtgac gcagtttgcg cagcgccttc gcttcgatct gacggatacg
      121 ttcgcgggta acgccgaact gtttacccac ttcttccagc gtgtagtcgg tgttcatatc
      181 gataccgaaa cgcatacgca gaacttttgc ttcacgcgcg gtcaggccag ccagcacgtc
      241 gtgcgttgcc gcacgcaggc tttcggtggt cgcagaatcc agcggcagct cgagctctgg
      301 tttctcttct ttcacttcag gctgacgtac atcacgtaag tcaacgaaag cttccagttg
      361 ttcagccaga atggttgccg cacgacgaat cgcctcttca ggatcgattg tgccgttggt
      421 ttccatttcg atgaccagct tgtccaggtc ggtacgctgt tctacacgcg ctgcttcaac
      481 attgtaggca atacgctcca cagggctgta gcatgcgtcg accagcagac ggccgattgg
      541 gcgctcatct tcttccgaat gaattcgggt agaagccggc acataaccac gaccgcgctg
      601 aactttgata cgcatgctaa tagacgcgtt ctcatcggtc aggtggcaga tcacgtgctg
      661 cggcttgacg atttcgacat caccgtcgtg ggtgatatcg gctgcagtca cagggccaat
      721 gccagattta ttcaaggtaa gaataacttc atctttgccc tgaactctca ccgccagccc
      781 tttcaggt
//
