LOCUS       sequence-87678-e        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human CTR1 from blasticidin resistance retroviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pWZLblasti-CTR1
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(731..748)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     958..977
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 tttatccagc cctcactcct tctctaggcg ccggccggat ccgccgccac catggatcat
       61 tcccaccata tggggatgag ctatatggac tccaacagta ccatgcaacc ttctcaccat
      121 cacccaacca cttcagcctc acactcccat ggtggaggag acagcagcgt gatgatgatg
      181 cctatgacct tctactttgg ctttaagaat gtggaactac tgttttccgg tttggtgatc
      241 aatacagctg gagaaatggc tggagctttt gtggcagtgt ttttactagc aatgttctat
      301 gaaggactca agatagcccg agagagcctg ctgcgtaagt cacaagtcag cattcgctac
      361 aattccatgc ctgtcccagg accaaatgga accatcctta tggagacaca caaaactgtt
      421 gggcaacaga tgctgagctt tcctcacctc ctgcaaacag tgctgcacat catccaggtg
      481 gtcataagct acttcctcat gctcatcttc atgacctaca acgggtacct ctgcattgca
      541 gtagcagcag gggccggtac aggatacttc ctcttcagct ggaagaaggc agtggtagtg
      601 gatatcacag agcattgcca ttgagtcgac caattccggt tattttccac catattgccg
      661 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
      721 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
      781 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
      841 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
      901 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
      961 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     1021 ggatctgatc tggggcctcg gtgcaca
//
