LOCUS       sequence-87683-e        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA tagged human ERK2 R67S from blasticidin resistance 
            retroviral vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pWZLblasti-HA-ERK2 R67S
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             83..109
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atcctccctt tatccagccc tcactccttc tctaggcgcc ggccggatcc agaattcact
       61 cgaggatatc gccgccacca tgtacccata cgatgttcca gattacgctg cggcggcggc
      121 ggcggcgggc gcgggcccgg agatggtccg cgggcaggtg ttcgacgtgg ggccgcgcta
      181 caccaacctc tcgtacatcg gcgagggcgc ctacggcatg gtgtgctctg cttatgataa
      241 tgtcaacaaa gttcgagtag ctatcaagaa aatcagcccc tttgagcacc agacctactg
      301 ccagagtacc ctgagggaga taaaaatctt actgcgcttc agacatgaga acatcattgg
      361 aatcaatgac attattcgag caccaaccat cgagcaaatg aaagatgtat atatagtaca
      421 ggacctcatg gaaacagatc tttacaagct cttgaagaca caacacctca gcaatgacca
      481 tatctgctat tttctctacc agatcctcag agggttaaaa tatatccatt cagctaacgt
      541 tctgcaccgt gacctcaagc cttccaacct gctgctcaac accacctgtg atctcaagat
      601 ctgtgacttt ggcctggccc gtgttgcaga tccagaccat gatcacacag ggttcctgac
      661 agaatatgtg gccacacgtt ggtacagggc tccagaaatt atgttgaatt ccaagggcta
      721 caccaagtcc attgatattt ggtctgtagg ctgcattctg gcagaaatgc tttctaacag
      781 gcccatcttt ccagggaagc attatcttga ccagctgaac cacattttgg gtattcttgg
      841 atccccatca caagaagacc tgaattgtat aataaattta aaagctagga actatttgct
      901 ttctcttcca cacaaaaata aggtgccatg gaacaggctg ttcccaaatg ctgactccaa
      961 agctctggac ttattggaca aaatgttgac attcaaccca cacaagagga ttgaagtaga
     1021 acaggctctg gcccacccat atctggagca gtattacgac ccgagtgacg agc
//
