LOCUS       sequence-87684-e         440 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of lambda-CI fused via three alanines to E. coli RNAP 
            beta subunit, aa 831-1057 (beta-flap). Can be cut with Not1 and 
            BamHI to replace beta-flap with a moiety of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Dove SL, Joung JK, Hochschild A
  TITLE     Activation of prokaryotic transcription through arbitrary 
            protein-protein contacts.
  JOURNAL   Nature. 1997 Apr 10;386(6625):627-30.
  PUBMED    9121589
REFERENCE   2  (bases 1 to 440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "1997-04-10"; volume: "386(6625)"; pages: "627-30"
COMMENT     Sequence Label: pAC-lambda-CI-beta-flap (831-1057)
FEATURES             Location/Qualifiers
     source          1..440
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tggcttatcc caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc
       61 tttatttaat ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct
      121 caaagttagc gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga
      181 agcggttagt atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt
      241 tcaggcaggg atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg
      301 ggtaagcaca accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc
      361 catgaccgca ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga
      421 ccctgagcag gctgttgagc
//
