LOCUS       sequence-87686-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI E-F HSP-T
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(72..320)
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(356..373)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(356..372)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(365..387)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(581..600)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     700..722
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(760..778)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agcttgcatg caggcctctg cagtcgacgg gcccgggatc cgatatctag atgcattcga
       61 ggtctctctc acttatcttt aatcatattc catagtccat accatagcac atacagtagt
      121 tatatgctgc agaagagatc caacaaaaca ttcacaatgg attatagaaa catttgttta
      181 ttcattataa tgagatctta cattcattta atattagaaa aagccacaaa ttcataacac
      241 aacaagccaa gaaaaaaaca caaacttaag cacacaagct ttttatttga cacaccaaat
      301 atttcatctt catcttcata tgatttgaga ccatcgaggt accgagctcg aattcactgg
      361 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      421 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      481 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
      541 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
      601 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      661 tgctcccggc atccgcttac agacaagctg tgaccttctc cgggagctgc atgtgtcaga
      721 ggttttcacc gtcatcaccg aaacgcgcga gacaaaaggg cctcgtgata cgcctatttt
      781 tataggttaa tgtcatgagc ggcgttgtga caatttaccg aacaactccg cggccgggaa
      841 gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat
      901 cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc
      961 tgcttg
//
