LOCUS       sequence-87692-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used
            as two-hybrid control. For cloning purposes, see Addgene plasmid 
            53734. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Dove SL, Joung JK, Hochschild A
  TITLE     Activation of prokaryotic transcription through arbitrary 
            protein-protein contacts.
  JOURNAL   Nature. 1997 Apr 10;386(6625):627-30.
  PUBMED    9121589
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "1997-04-10"; volume: "386(6625)"; pages: "627-30"
COMMENT     Sequence Label: pBR-alpha
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     94..113
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcccaanagt cccccggcca cggggnctgc caccataccc acgccgaaac aagcgctcat
       61 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
      121 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatccttac
      181 tcgtcagcga tgcttgccgg tggccagttt tccaggcgca tgcccagaga cagtccacgg
      241 gaagccagca cgtctttaat ctcagtaaga gattttttac caaggttagg cgttttaagg
      301 agctcaacct cggtacgctg taccagatca ccgatatagt ggatagcttc tgctttaagg
      361 cagttagcag agcggacagt caattccaga tcgtcaacag ggcgcagcag gatcggatcg
      421 aactctggtt tctcttcttt cacttcaggc tgacgtacat cacgtaagtc aacgaaagct
      481 tccagttgtt cagccagaat ggttgccgca cgacgaatcg cctcttcagg atcgattgtg
      541 ccgttggttt ccatttcgat gaccagcttg tccaggtcgg tacgctgttc tacacgcgct
      601 gcttcaacat tgtaggcaat acgctccaca gggctgtagc atgcgtcgac cagcagacgg
      661 ccgattgggc gctcatcttc ttccgaatga attcgggtag aagccggcac ataaccacga
      721 ccgcgctgaa ctttgatacg
//
