LOCUS       sequence-87700-e         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI Intron c1
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(404..421)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(404..420)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(413..435)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(629..648)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     748..770
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(808..826)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agcttgcatg caggcctctg cagtcgacgg gcccgggatc cgatatctag atgcattcga
       61 tggtctcaaa ggaattcctg tggttggaga agctagaacc tgcaaaacaa tcccaagaat
      121 tgttcagact tcaagaaaag agacaaatag caataacata attaagaaag agtcgagagt
      181 tgactctaaa agtctttctc tctctttgaa accgttgacc cataacttta tcacttccga
      241 tcacgattca ttgtcaagat gttaaatcaa tgatacatca aacacagatt atcatcacta
      301 attggattca tttcaaaaga aacagagaaa aaaaacagag aggcttacgt tagcagagga
      361 ggagaagctc cttagagacc tacgaggtac cgagctcgaa ttcactggcc gtcgttttac
      421 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
      481 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
      541 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
      601 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
      661 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
      721 ccgcttacag acaagctgtg accttctccg ggagctgcat gtgtcagagg ttttcaccgt
      781 catcaccgaa acgcgcgaga caaaagggcc tcgtgatacg cctattttta taggttaatg
      841 tcatgagcgg cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc
      901 ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg
      961 tatgcccaac tttgtataga gagccactgc gggatcgtc
//
