LOCUS       sequence-87702-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of alpha NTD fused via three alanines to E. coli RNAP 
            subunit beta, aa 831-1057 (beta-flap). Can be cut with Not1 and 
            BamHI to replace beta-flap with a moiety of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Dove SL, Joung JK, Hochschild A
  TITLE     Activation of prokaryotic transcription through arbitrary 
            protein-protein contacts.
  JOURNAL   Nature. 1997 Apr 10;386(6625):627-30.
  PUBMED    9121589
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "1997-04-10"; volume: "386(6625)"; pages: "627-30"
COMMENT     Sequence Label: pBR-alpha-beta flap (831-1057)
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 actctagacc aaagagagga cacaatgcag ggttctgtga cagagtttct aaaaccgcgc
       61 ctggttgata tcgagcaagt gagttcgacg cacgccaagg tgacccttga gcctttagag
      121 cgtggctttg gccatactct gggtaacgca ctgcgccgta ttctgctctc atcgatgccg
      181 ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac atgagtacag caccaaagaa
      241 ggcgttcagg aagatatcct ggaaatcctg ctcaacctga aagggctggc ggtgagagtt
      301 cagggcaaag atgaagttat tcttaccttg aataaatctg gcattggccc tgtgactgca
      361 gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc cgcagcacgt gatctgccac
      421 ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag ttcagcgcgg tcgtggttat
      481 gtgccggctt ctacccgaat tcattcggaa gaagatgagc gcccaatcgg ccgtctgctg
      541 gtcgacgcat gctacagccc tgtggagcgt attgcctaca atgttgaagc agcgcgtgta
      601 gaacagcgta ccgacctgga caagctggtc atcgaaatgg aaaccaacgg cacaatcgat
      661 cctgaagagg cgattcgtcg tgcggcaacc attctggctg aacaactgga agctttcgtt
      721 gacttacgtg atgtacgtca gcctgaagtg aaagaagaga aaccagaggc ggccgcaatc
      781 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tgggtccgga agagatcacc
      841 gctga
//
