LOCUS       sequence-87710-e        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI F-G neo c2
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        129..308
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        161..344
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     complement(419..438)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1029..1048
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 agcttgcatg caggcctctg cagtcgacgg gcccggtctc ttgaggatca tgagcggaga
       61 attaagggag tcacgttatg acccccgccg atgacgcggg acaagccgtt ttacgtttgg
      121 aactgacaga accgcaacgt tgaaggagcc actcagccgc gggtttctgg agtttaatga
      181 gctaagcaca tacgtcagaa accattattg cgcgttcaaa agtcgcctaa ggtcactatc
      241 agctagcaaa tatttcttgt caaaaatgct ccactgacgt tccataaatt cccctcggta
      301 tccaattaga gtctcatatt cactctcaat ccaaataatc tgcaccggat ctggatcgtt
      361 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
      421 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
      481 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
      541 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
      601 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
      661 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
      721 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
      781 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
      841 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
      901 cgacggcgat gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
      961 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     1021 ggacatagcg ttggctaccc gtgatattgc tga
//
