LOCUS       sequence-87725-e         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA tagged human ERK2 R67SD321N from blasticidin resistance
            retroviral vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pWZLblasti-HA-ERK2 GOF
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..59)
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
ORIGIN
        1 aataaccgga attggtcgac ttaagatctg tatcctggct ggaatctagc agtctcttca
       61 aaaattagtt ctttgagctt ttccttaggc aagtcatcca attccatgtc gaacttgaat
      121 ggtgcttcgg cgatgggctc gttactactc gggtcgtaat actgctccag atatgggtgg
      181 gccagagcct gttctacttc aatcctcttg tgtgggttga atgtcaacat tttgtccaat
      241 aagtccagag ctttggagtc agcatttggg aacagcctgt tccatggcac cttatttttg
      301 tgtggaagag aaagcaaata gttcctagct tttaaattta ttatacaatt caggtcttct
      361 tgtgatgggg atccaagaat acccaaaatg tggttcagct ggtcaagata atgcttccct
      421 ggaaagatgg gcctgttaga aagcatttct gccagaatgc agcctacaga ccaaatatca
      481 atggacttgg tgtagccctt ggaattcaac ataatttctg gagccctgta ccaacgtgtg
      541 gccacatatt ctgtcaggaa ccctgtgtga tcatggtctg gatctgcaac acgggccagg
      601 ccaaagtcac agatcttgag atcacaggtg gtgttgagca gcaggttgga aggcttgagg
      661 tcacggtgca gaacgttagc tgaatggata tattttaacc ctctgaggat ctggtagaga
      721 aaatagcaga tatggtcatt gctgaggtgt tgtgtcttca agagcttgta aagatctgtt
      781 tccatgaggt cctgtactat atatacatct ttcatttgct cgatggttgg tgctcgaata
      841 atgtcattga ttccaatgat gttctcatgt ctgaagcgca gtaagatttt tatctccctc
      901 agggtactct ggcagtaggt ctggtgctca aaggggctga ttttcttgat agctactcga
      961 actttgttga c
//
