LOCUS       sequence-87733-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIc R 3-4
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    151..275
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(316..621)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(418..437)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ctatagggcg aattggcgga aggccgtcaa ggccacgtgt cttgtccagg cgcgccatga
       61 gctctaaagc ttcgctcgag ctgcggccgc acggatcctc gaattcccaa gcttatctcg
      121 acccgaagac aagacagcgg tgagacccca accactttgt acaagaaagc tgaacgagaa
      181 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
      241 taatactgta aaacacaaca tatccagtca ctatggtcga cctgcagact ggctgtgtat
      301 aagggagcct gacatttata ttccccagaa catcaggtta atggcgtttt tgatgtcatt
      361 ttcgcggtgg ctgagatcag ccacttcttc cccgataacg gaaaccggca cactggccat
      421 atcggtggtc atcatgcgcc agctttcatc cccgatatgc accaccgggt aaagttcacg
      481 ggggacttta tctgacagca gacgtgcact ggccaggggg atcaccatcc gtcgcccggg
      541 cgtgtcaata atatcactct gtacatccac aaacagacga taacggctct ctcttttata
      601 ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc gtcagcaaaa gagccgttca
      661 tttcaataaa ccgggcgacc tcagccatcc cttcctgatt ttccgctttc cagcgttcgg
      721 cacgcagacg acgggcttca ttctgcatgg ttgtgcttac cagaccggag atattgacat
      781 catatatgcc ttgagcaact gatagctgtc gctgtcaact gtcactgtaa tacgctgctt
      841 catagcatac ctctttttga catacttcgg gtatacatat cagtatatat tcttataccg
      901 caaaaatcag cgcgcaaata cgcatactgt tatctggctt ttagtaag
//
