LOCUS       sequence-87734-e         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIc F 5-6
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    161..285
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(326..631)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(428..447)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 atacgactca ctatagggcg aattggcgga aggccgtcaa ggccacgtgt cttgtccagg
       61 cgcgccatga gctctaaagc ttcgctcgag ctgcggccgc acggatcctc gaattcccaa
      121 gcttatctcg acccgaagac aacagagaca tgagacccca accactttgt acaagaaagc
      181 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
      241 acagactaca taatactgta aaacacaaca tatccagtca ctatggtcga cctgcagact
      301 ggctgtgtat aagggagcct gacatttata ttccccagaa catcaggtta atggcgtttt
      361 tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc cccgataacg gaaaccggca
      421 cactggccat atcggtggtc atcatgcgcc agctttcatc cccgatatgc accaccgggt
      481 aaagttcacg ggggacttta tctgacagca gacgtgcact ggccaggggg atcaccatcc
      541 gtcgcccggg cgtgtcaata atatcactct gtacatccac aaacagacga taacggctct
      601 ctcttttata ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc gtcagcaaaa
      661 gagccgttca tttcaataaa ccgggcgacc tcagccatcc cttcctgatt ttccgctttc
      721 cagcgttcgg cacgcagacg acgggcttca ttctgcatgg ttgtgcttac cagaccggag
      781 atattgacat catatatgcc ttgagcaact gatagctgtc gctgtcaact gtcactgtaa
      841 tacgctgctt catagcatac ctctttttga catacttcgg gtatacatat cagtatatat
      901 tcttataccg caaaaatcag cgcgcaaata cgcatactgt tatctggctt ttagtaagcc
      961 ggatcctcta gattacgccc cgccctgcc
//
