LOCUS       sequence-87735-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIc F 1-2 RNAi
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(454..759)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(556..575)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 atacgactca ctatagggcg aattggcgga aggccgtcaa ggccacgtgt cttgtccagg
       61 cgcgccatga gctctaaagc ttcgctcgag ctgcggccgc acggatcctc gaattcccaa
      121 gcttatctcg acccgaagac aagcccgaca ctaggctagc ttaggtcact caaggtcact
      181 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
      241 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataagaa ccctaattcc
      301 cttatctggg aactactcac acattattct ggagaaaaat agagagagat agatttgtag
      361 agagagactg gtgatttttg cggactctag catggccgga ttcacctgag accgtcgacc
      421 tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat
      481 ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga
      541 aaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac
      601 caccgggtaa agttcacggg ggactttatc tgacagcaga cgtgcactgg ccagggggat
      661 caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata
      721 acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt
      781 cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt
      841 ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca
      901 ga
//
