LOCUS       sequence-87736-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIc F 3-4
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    157..281
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(318..323,324..341,342..348)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(468..487)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tacgactcac tatagggcga attggcggaa ggccgtcaag gccacgtgtc ttgtccaggc
       61 gcgccatgag ctctaaagct tcgctcgagc tgcggccgca cggatcctcg aattcccaag
      121 cttatctcga cccgaagaca aattggcggt gagaccacaa gtttgtacaa aaaagctgaa
      181 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
      241 actacataat actgtaaaac acaacatatc cagtcactat ggcggcccct cgaggggccg
      301 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
      361 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
      421 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
      481 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
      541 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
      601 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
      661 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
      721 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
      781 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
      841 ccaatccctg ggtgagtttc acc
//
