LOCUS       sequence-87737-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIc F 2-3
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    155..279
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(316..321,322..339,340..346)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(466..485)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgactcacta tagggcgaat tggcggaagg ccgtcaaggc cacgtgtctt gtccaggcgc
       61 gccatgagct ctaaagcttc gctcgagctg cggccgcacg gatcctcgaa ttcccaagct
      121 tatctcgacc cgaagacaag ggcgcggtga gaccacaagt ttgtacaaaa aagctgaacg
      181 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
      241 tacataatac tgtaaaacac aacatatcca gtcactatgg cggcccctcg aggggccgca
      301 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt
      361 taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
      421 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
      481 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
      541 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
      601 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt
      661 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
      721 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
      781 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctca
//
